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Eco-Friendly and Temperature Dependent Biosynthesis of Gold Nanoparticles Using the Bacterium Pseudomonas aeruginosa: Characterization and Antibacterial Activity

机译:铜绿假单胞菌细菌的金纳米颗粒的生态友好和温度依赖性生物合成:表征和抗菌活性。

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In this paper we report the biological synthesis of gold nanoparticles (GNPs) by the reduction of gold ions using a suspension and supernatant of P. aeruginosa. The biosynthesis method was straightforward and yielded good results without using toxic chemicals. The size distribution of the gold nanoparticles synthesized by P. aeruginosa at higher temperatures was larger than that synthesized at lower temperatures. The GNPs morphology was isotropic at various temperatures. With an increase in the temperature, the stability of the GNPs decreased. The absorption and fluorescence spectra accorded well with the size distribution of the particles, with the nanoparticle size increasing as the absorption and fluorescence increased too. The optical properties of the GNPs observed in the study accorded well with the scanning electron microscopy (SEM) observations. The visible photoluminescence (PL) around 435 nm indicated the possible use of the obtained colloids, which consisted of GNPs and capping biomaterial, in therapeutic applications. Moreover, the synthesized GNPs showed good antibacterial activity toward E. coli indicating their potential in biological applications.
机译:在本文中,我们报道了通过铜绿假单胞菌的悬浮液和上清液通过还原金离子来生物合成金纳米颗粒(GNP)。该生物合成方法简单易行,并且在不使用有毒化学物质的情况下产生了良好的效果。铜绿假单胞菌在较高温度下合成的金纳米颗粒的尺寸分布大于在较低温度下合成的金纳米颗粒的尺寸分布。在不同温度下,GNPs形态各向同性。随着温度的升高,GNP的稳定性降低。吸收光谱和荧光光谱与颗粒的尺寸分布非常吻合,纳米颗粒的尺寸也随着吸收光谱和荧光的增加而增加。在这项研究中观察到的GNP的光学特性与扫描电子显微镜(SEM)的观察非常吻合。约435 nm的可见光致发光(PL)表明,由GNP和加盖生物材料组成的胶体在治疗应用中的可能用途。此外,合成的GNP对大肠杆菌显示出良好的抗菌活性,表明它们在生物应用中具有潜力。

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