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首页> 外文期刊>Journal of oceanography >Variability in the production of organic ligands, by Synechococcus PCC 7002, under different iron scenarios
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Variability in the production of organic ligands, by Synechococcus PCC 7002, under different iron scenarios

机译:Synechococcus PCC 7002在不同铁情景下生产有机配体的可变性

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Several Fe-uptake mechanisms suggest the importance of the presence of certain organic ligands in phytoplankton exudates. Here, it has been studied how Synechococcus (strain PCC 7002) acclimates to Fe-bioavailability, comparing the growth and organic exudation under two different Fe regimes. These cyanobacteria were incubated in UV-treated seawater supplemented only with major nutrients and two different iron scenarios (Low-Fe and High-Fe), without chelating agents, in order to analyze the organic ligands production. The levels of dissolved organic carbon (DOC) and two natural ligands (hydroxamic and phenolic moieties) were monitored. The responses in the organic extracellular release rates (ER), normalized per cell, were statistically analyzed considering Fe scenarios and different development stages. Growth of Synechococcus was significantly slower under Low-Fe treatment, suggesting that these cultures were iron limited compared to those flourished with higher levels of iron in the medium. Although the concentration of DOC increased to 127.13 +/- 8.38 and 150.51 +/- 8.59 mu mol C L-1 under Low-Fe and High-Fe conditions, respectively, no-significant variations were found in the DOCER, among growth phases and iron bioavailability scenarios. Under High-Fe conditions, the production of hydroxamic ligands was inhibited, while the extracellular release rate of phenolic compounds decreased, regarding to Low-Fe conditions. Growth phases of Synechococcus also modified the extracellular release rates both of hydroxamic and phenolic moieties. The present study, therefore, demonstrates that iron availability and growth stages might be key parameters in regulating the release performance of extracellular Fe-specific organic ligands by cyanobacteria.
机译:几种铁吸收机制表明浮游植物渗出物中某些有机配体的存在很重要。在这里,已经研究了Synechococcus(PCC 7002菌株)如何适应Fe的生物利用度,比较了两种不同Fe体制下的生长和有机渗出。这些蓝细菌在不添加螯合剂的情况下,在仅添加主要营养素和两种不同铁元素(低铁和高铁)的情况下,在经过紫外线处理的海水中孵育,以分析有机配体的产生。监测溶解有机碳(DOC)和两个天然配体(异羟肟酸和酚部分)的水平。考虑到铁的情况和不同的发育阶段,对每个细胞标准化的有机细胞外释放速率(ER)的响应进行了统计分析。在低铁处理下,合成球菌的生长明显减慢,这表明与培养基中铁含量较高的培养基相比,这些培养基的铁含量有限。尽管在低铁和高铁条件下DOC的浓度分别增加到127.13 +/- 8.38和150.51 +/- 8.59μmol C L-1,但是在DOCER中,在生长期和生长期之间,没有发现明显的变化。铁的生物利用度方案。在高铁条件下,相对于低铁条件,异羟肟酸配体的产生受到抑制,而酚类化合物的胞外释放速率降低。 Synechococcus的生长期也改变了异羟肟酸和酚部分的细胞外释放速率。因此,本研究表明,铁的有效性和生长阶段可能是调节蓝藻胞外铁特异性有机配体释放性能的关键参数。

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