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Un-polarized light transmission DIC microscope

机译:无偏振光透射DIC显微镜

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摘要

A new technique for creating Differential Interference Contrast (DIC) images of transparent specimens, using un-polarized, white light is discussed. A Sagnac interferometer (SI) is used in the image space of a transmission DIC microscope to produce small/differential laterally sheared, coherent beam components that interfere to form DIC images. Phase shifts between the exit beam components are introduced by applying small angular rotation of the SI in its own plane. Results obtained for a transparent watermark on a glass slide are presented. Most important advantage of the technique is that the birefringence of a test specimen does not affect the DIC images as un-polarized light is used.
机译:讨论了一种使用非偏振白光创建透明标本的微分干涉对比度(DIC)图像的新技术。在透射DIC显微镜的图像空间中使用Sagnac干涉仪(SI),以产生小的/差分横向剪切的相干光束分量,这些分量会干扰形成DIC图像。通过在SI自身平面中施加较小的SI角旋转,可以在出射光束分量之间引入相移。给出了在载玻片上获得透明水印的结果。该技术最重要的优点是,由于使用了非偏振光,因此试样的双折射不会影响DIC图像。

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