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A molecular study on Theileria and Babesia in cattle from Isfahan province, Central Iran

机译:伊朗中部伊斯法罕省牛中的泰勒虫和巴比虫的分子研究

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摘要

Bovine theileriosis and babesiosis are important hemoprotozoal diseases of cattle in tropical and subtropical regions that lead to economic losses in these animals. From March 2009 to July 2009, 176 blood samples of Holstein and crossbred cattle without any signs of disease were prepared from Isfahan province, Central Iran. The extracted DNA from blood cells were analyzed for members of the genera Theileria and Babesia by polymerase chain reaction (PCR) using a set of primers derived from the 18s rRNA gene. 42 out of 176 blood samples (23.9 %) were positive for Theileria spp. and none of them was positive for Babesia spp.. The present study showed that Theileria is detectable in cattle without any sign of infection but maintained a persistant sub-clinical state in the carrier cattle, which can serve as reservoirs of infection for ticks and cause natural transmission of the disease.
机译:在热带和亚热带地区,牛回肠虫病和巴贝虫病是重要的牛血虫病,导致这些动物的经济损失。从2009年3月至2009年7月,从伊朗中部伊斯法罕省采集了176份无任何疾病迹象的荷斯坦和杂交牛的血液样本。使用一组源自18s rRNA基因的引物,通过聚合酶链反应(PCR)对从血细胞中提取的DNA进行泰勒氏菌和巴贝斯虫属成员的分析。 176个血液样本中有42个(23.9%)的泰勒菌属呈阳性。本研究表明在牛中可检测到泰勒菌属,无任何感染迹象,但在携带牛中仍保持持久的亚临床状态,可作为s和病原体的感染源。疾病的自然传播。

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