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首页> 外文期刊>Journal of Parasitic Diseases >Protective immunity using MPL-A and autoclaved Leishmanian donovani as adjuvants along with a cocktail vaccine in murine model of visceral leishmaniasis
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Protective immunity using MPL-A and autoclaved Leishmanian donovani as adjuvants along with a cocktail vaccine in murine model of visceral leishmaniasis

机译：在内脏利什曼病鼠模型中使用MPL-A和高压灭菌的利什曼原虫作为佐剂和鸡尾酒疫苗的保​​护性免疫

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摘要

The current study is an extension of our previous study where we tested the protective efficacy of gp63 and Hsp70 against murine visceral leishmaniasis. The cocktail vaccine was combined with MPL-A and ALD adjuvants and the protection afforded by the three vaccines was compared. Inbred BALB/c mice were immunized twice at an interval of two weeks with the vaccine formulations. Two weeks after the booster, they were challenged with 107 promastigotes of Leishmania donovani and sacrificed on 30, 60 and 90 days post infection/challenge. The protective efficacy of vaccines was analyzed by assessment of the hepatic and splenic parasite burden and generation of cellular and humoral immune responses. The immunized animals revealed a significant reduction in parasite burden as compared to the infected controls. These animals also showed heightened DTH response, increased generation of IgG2a, IFN-γ and IL-2 by spleen cells. This was also accompanied by a decrease in the levels of IgG1 and IL-10. Mice immunized with gp63+Hsp70+MPL-A exhibited significantly greater protection in comparison to those immunized with gp63+Hsp70+ALD.
机译：当前的研究是我们先前研究的扩展，其中我们测试了gp63和Hsp70对小鼠内脏利什曼病的保护作用。将鸡尾酒疫苗与MPL-A和ALD佐剂混合使用，并比较了这三种疫苗提供的保护作用。将近交BALB / c小鼠以两周的间隔用疫苗制剂免疫两次。加强免疫后两周，他们受到107个前鞭毛雷什曼原虫的攻击，并在感染/攻击后30、60和90天处死。通过评估肝脏和脾脏寄生虫负担以及细胞和体液免疫反应的产生来分析疫苗的保护功效。与感染的对照相比，免疫的动物显示寄生虫负担显着降低。这些动物还表现出增强的DTH反应，脾细胞产生的IgG2a，IFN-γ和IL-2增多。这还伴随着IgG1和IL-10水平的降低。与用gp63 + Hsp70 + ALD免疫的小鼠相比，用gp63 + Hsp70 + MPL-A免疫的小鼠表现出明显更大的保护作用。

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