首页> 外文期刊>Journal of Parasitic Diseases >Soil moisture effects on the activity of three entomopathogenic nematodes (Steinernematidae and Heterorhabditidae) isolated from Meghalaya, India
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Soil moisture effects on the activity of three entomopathogenic nematodes (Steinernematidae and Heterorhabditidae) isolated from Meghalaya, India

机译:土壤水分对印度梅加拉亚邦三种昆虫致病性线虫(Steinernematidae和Heterorhabditidae)活性的影响

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摘要

Entomopathogenic nematodes (EPNs) are obligate parasites of insects that are widely distributed in soils throughout the world. They have great potential for use as biological control agents for insect pests. It is known that strains of Steinernema and Heterorhabditis isolated from different geographical regions exhibit differences in their ecological traits, such as infectivity, establishment, survival, reproduction, etc. A precise knowledge of these factors is therefore an essential pre-requisite for devising successful strategies to use these nematodes in biological control programmes. The present study investigated the effect of soil moisture on the activity (as measured by number of nematodes established in hosts) of three entomopathogenic nematode species (Heterorhabditis indica Poinar, Karunakar & David; Steinernema thermophilum Ganguly & Singh; Steinernema glaseri Steiner), isolated from forest soils in Meghalaya, India, under laboratory conditions. The experiments for EPNs were conducted at 25 ± 2°C (30 ± 2°C for S. thermophilum) in a sandy loam soil (85% sand, 12% silt and 3% clay, pH 6.54). Last instar larvae of wax moth, Galleria mellonella served as the experimental insect host. The soil moistures tested were 3, 4, 5, 6, 8, 10, 12, 14, 16, 18, 20, 22 and 25% (w/w). The study revealed that soil moisture has marked influences on establishment of infective juveniles of different nematode species in insect host. While, S. thermophilum showed establishment at 4% and above soil moistures, H. indica and S. glaseri showed establishment at 5% and above soil moistures. The optimum soil moisture for different nematode species were noted as: H. indica 8–18%, S. thermophilum 6–20%, and S. glaseri 8–25%. Further, a minimum of 6% soil moisture was noted to be essential for achieving 100% host mortality for all the three nematode species.
机译:昆虫病原线虫(EPN)是专性的昆虫寄生虫,广泛分布于世界各地的土壤中。它们具有用作虫害生物防治剂的巨大潜力。众所周知,分离自不同地理区域的斯坦氏湿疣和异种性哈氏菌菌株在生态特征(如传染性,建立,存活,繁殖等)上表现出差异。因此,对这些因素的准确了解是制定成功策略的必要先决条件在生物防治计划中使用这些线虫。本研究调查了土壤水分对三种昆虫致病性线虫物种(印度Heterorhabditis inin Poinar,Karunakar和David; Steinernema thermophilum Ganguly和Singh; Steinernema glaseri Steiner)的活性(以寄主中建立的线虫数量衡量)的影响。在实验室条件下,印度梅加拉亚邦的森林土壤。 EPNs的实验是在沙质壤土(85%的沙子,12%的淤泥和3%的粘土,pH 6.54)中于25±2°C(嗜热链球菌为30±2°C)进行的。蜡蛾的最后一龄幼虫,Malleria mellonella作为实验昆虫宿主。测试的土壤水分为3、4、5、6、8、10、12、14、16、18、20、22和25%(w / w)。研究表明,土壤水分对昆虫寄主中不同线虫物种的感染性幼虫的建立有显着影响。而嗜热链球菌在土壤水分中的含量高于或等于4%,而H稻和格拉希里葡萄菌在土壤水分中的含量高于或等于5%。不同线虫种类的最佳土壤水分为:H链球菌8–18%,嗜热链球菌6–20%和格拉索里链霉菌8–25%。此外,对于所有三种线虫物种,要达到100%的寄主死亡率,至少要有6%的土壤湿度是至关重要的。

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