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首页> 外文期刊>Journal of Parasitology >REDUCED HAMSTER USAGE AND STRESS IN PROPAGATING LEISHMANIA CHAGASI PROMASTIGOTES USING CRYOPRESERVATION AND SAPHENOUS VEIN INOCULATION
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REDUCED HAMSTER USAGE AND STRESS IN PROPAGATING LEISHMANIA CHAGASI PROMASTIGOTES USING CRYOPRESERVATION AND SAPHENOUS VEIN INOCULATION

机译：冷冻保存和高脂静脉接种在减少利什曼原虫传播中减少仓鼠的使用和应力

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摘要

Leishmania chagasi, a causal agent of visceral leishmaniasis, requires passage through lab animals such as hamsters to maintain its virulence. Hamster infection is typically accomplished via cardiac puncture or intraperitoneal injection, procedures accompanied by risks of increased animal stress and death. The use of the hamster model also necessitates a regular supply of infected animals, because L. chagasi parasites newly isolated from an infected hamster can be grown in culture for only several weeks before loss of function/phenotype occurs. In an effort to decrease animal usage and animal stress, experiments were performed to assess a more gentle inoculation procedure (saphenous vein inoculation) and the use of cryopreserved parasite cells for research experiments. Of 81 hamsters inoculated by the saphenous vein, 80 became infected as determined ante mortem, by display of clinical symptoms of leishmaniasis (onset of symptoms at 105 ± 22 days post-inoculation), and postmortem by the presence of parasites within the spleen. Splenic parasite load calculated for a subset (n = 34) of infected hamsters was 124 to 26,177 Leishmania donovani infection units. Cryopreserved, and never-stored, cells were equivalent in all properties evaluated, including developmental changes in morphology during culture, culture growth rates, parasite resistance to serum-mediated lysis, and expression of developmentally regulated surface proteins major surface protease and promastigote surface antigen.
机译：利什曼原虫是内脏利什曼病的病原体，需要通过仓鼠等实验动物来维持其毒性。仓鼠感染通常通过心脏穿刺或腹膜内注射来完成，伴随着增加动物压力和死亡的风险。使用仓鼠模型还需要定期供应受感染的动物，因为从受感染的仓鼠中新分离出的南美锥虫寄生虫只能在培养中生长数周，然后才能丧失功能/表型。为了减少动物的使用和动物的压力，进行了实验以评估更温和的接种程序（大隐静脉接种），并使用冷冻保存的寄生虫细胞进行研究实验。在隐性大隐静脉接种的81只仓鼠中，有80只在确定的死后被感染，表现为利什曼病的临床症状（接种后105±22天开始出现症状），死后在脾脏中存在寄生虫。为感染的仓鼠的一个子集（n = 34）计算的脾寄生虫负荷为124到26,177利什曼原虫多诺万尼感染单位。冷冻保存且从未保存过的细胞在所有评估的特性中都是等效的，包括培养过程中形态的发育变化，培养物生长速率，对血清介导的裂解的寄生虫抗性以及受发育调节的表面蛋白主要表面蛋白酶和前鞭毛体表面抗原的表达。

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