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首页> 外文期刊>Journal of Parasitology >CHARACTERIZATION OF DICTYOCAULUS SPECIES (NEMATODA: TRICHOSTRONGYLOIDEA) FROM THREE SPECIES OF WILD RUMINANTS IN NORTHWESTERN SPAIN
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CHARACTERIZATION OF DICTYOCAULUS SPECIES (NEMATODA: TRICHOSTRONGYLOIDEA) FROM THREE SPECIES OF WILD RUMINANTS IN NORTHWESTERN SPAIN

机译：从西北地区的三种野生反刍动物中鉴定金丝藻的种类（线虫：线粒体）

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摘要

Specimens of Dictyocaulus spp. were extracted from the respiratory tracts of 3 ruminant hosts including roe deer (Capreolus capreolus), red deer (Cervus elaphus), and chamois (Rupicapra rupicapra) from wild populations in the province of León, northwestern Spain. The near-complete nuclear small-subunit ribosomal RNA gene, and 2 regions of the large-subunit ribosomal RNA gene, were amplified by PCR and sequenced. The SSU rDNA gene sequences indicated a high level of similarity between the isolate from C. elaphus and the published sequences for Dictyocaulus eckerti. SSU rDNA gene sequences were identical in the isolates from C. capreolus and R. rupicapra, and both corresponded to published sequences for D. capreolus. The LSU rDNA gene sequences differed in isolates from the latter 2 hosts, indicating the possible presence of an undescribed Dictyocaulus sp. in R. rupicapra. These results showed that the LSU rDNA gene sequences are useful indicators of genetic and species diversity in species of Dictyocaulus.
机译：Dictyocaulus spp。的标本提取自西班牙西北部莱昂省野生种群的3种反刍动物的呼吸道，包括hosts（Capreolus capreolus），马鹿（Cervus elaphus）和羚羊（Rupicapra rupicapra）。通过PCR扩增近完整的核小亚基核糖体RNA基因和大亚基核糖体RNA基因的2个区域。 SSU rDNA基因序列表明，来自C. elaphus的分离株与已出版的Dictyocaulus eckerti序列具有高度相似性。 SRE rDNA基因序列在来自毛孢假单胞菌和卢比罗非鱼的分离物中是相同的，并且都对应于已公开的毛孢假单胞菌的序列。 LSU rDNA基因序列在与后2个宿主的分离物中有所不同，表明可能存在未描述的Dictyocaulus sp。在R. rupicapra。这些结果表明，LSU rDNA基因序列是密齿ic物种遗传和物种多样性的有用指标。

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