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首页> 外文期刊>Journal of Parasitology >DISTINCT PHOSPHATASE ACTIVITY PROFILES IN TWO STRAINS OF TRYPANOSOMA CRUZI
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DISTINCT PHOSPHATASE ACTIVITY PROFILES IN TWO STRAINS OF TRYPANOSOMA CRUZI

机译：克鲁维氏酵母两株菌株的磷酸酶活性谱

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摘要

Phosphorylation of parasite proteins plays a key role in the process of cell invasion by Trypanosoma cruzi, the etiologic agent of Chagas' disease. In this sense, characterization of parasite kinases and phosphatases could open new possibilities for the rational design of chemotherapeutic agents for the treatment of Chagas' disease. In this work, we analyzed phosphatase activities in T. cruzi homogenates from 2 strains belonging to different lineages and with different resistance to oxidative stress. Tulahuen 2 cells (Lineage I) showed higher phosphatase activities and specificity constants when compared to the Y strain (Lineage II). Tulahuen 2 had an optimum phosphatase activity at pH 4.0 and the Y strain at pH 7.0. In both cases, neutral–basic, but not acid, phosphatase activities were increased in the presence of Mg2+. Although calcium had an inhibitory effect at a pH of 7.0 and 8.0 in the Y strain, this inhibition was restricted to pH 8.0 in the other strain. Different substrates and acid phosphotyrosine and alkaline phosphatase inhibitors exhibited distinct effects on the phosphatase activity of both strains. Our results provide a better understanding of T. cruzi phosphatases and reinforce the notion of heterogeneity among T. cruzi populations.
机译：恰加斯氏病的病原体克氏锥虫在细胞入侵过程中，寄生虫蛋白质的磷酸化起着关键作用。从这个意义上讲，寄生虫激酶和磷酸酶的表征可以为合理设计用于治疗恰加斯氏病的化学治疗药物开辟新的可能性。在这项工作中，我们分析了来自2个菌株的克鲁斯匀浆匀浆中的磷酸酶活性，这2个菌株属于不同谱系，并且对氧化应激的抵抗力不同。与Y菌株（谱系II）相比，Tulahuen 2细胞（谱系I）显示出更高的磷酸酶活性和特异性常数。 Tulahuen 2在pH 4.0时具有最佳磷酸酶活性，在pH 7.0时具有Y菌株。在两种情况下，存在Mg 2 + 时，中性碱性磷酸酶活性均升高，但不升高。尽管钙在Y菌株中的pH值为7.0和8.0时具有抑制作用，但在另一菌株中该抑制作用仅限于pH 8.0。不同的底物和酸性磷酸酪氨酸和碱性磷酸酶抑制剂对两种菌株的磷酸酶活性均表现出不同的影响。我们的结果提供了一个更好的了解克鲁姆氏菌磷酸酶，并加强了克鲁格氏菌种群之间的异质性概念。

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