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首页> 外文期刊>Journal of Parasitology >Inhibition of Interleukin-12 Production by Trypanosoma brucei in Rat Macrophages
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Inhibition of Interleukin-12 Production by Trypanosoma brucei in Rat Macrophages

机译：布氏锥虫对大鼠巨噬细胞白细胞介素12的抑制作用

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摘要

The immune response of a host infected with Trypanosoma brucei is modulated by trypomastigotes. We examined the changes in cytokine production in T. brucei gambiense (Wellcome strain; WS) infected rats and the influence on production of interleukin (IL)-12 by macrophages. The blood concentration of interferon-γ, tumor necrosis factor–α, and IL-10 increased beginning the second day after infection. However, an increase in IL-12p40 was not observed until 4 days after infection. When spleen macrophages and Kupffer cells harvested from uninfected rats and HS-P cells (a rat macrophagelike cell line) were cocultured with WS, IL-12p40 production did not change. When HS-P cells were cultured with WS, transport of nuclear factor– κB into the nucleus increased. Levels of macrophage colony-stimulating factor (M-CSF) and granulocyte macrophage colony-stimulating factor mRNA in the spleens and livers of WS-infected rats were high in comparison with uninfected rats, suggesting that the WS promotes macrophage proliferation. The level of IL-12p40 mRNA in HS-P cells cocultured with WS increased in response to transfection with a small interfering RNA against M-CSF or addition of anti–M-CSF antibody. These results suggest that the WS inhibits IL-12p40 mRNA production by promoting production of macrophage colony-stimulating factor by macrophages.
机译：锥虫感染了布鲁氏锥虫感染的宿主的免疫反应。我们检查了T. brucei gambiense（Wellcome株； WS）感染的大鼠中细胞因子产生的变化以及巨噬细胞对白介素（IL）-12产生的影响。感染后第二天开始，干扰素-γ，肿瘤坏死因子-α和IL-10的血药浓度增加。然而，直到感染后4天才观察到IL-12p40的增加。当从未感染的大鼠收获的脾巨噬细胞和库普弗细胞与HS-P细胞（一种大鼠巨噬细胞样细胞系）与WS共培养时，IL-12p40的产量没有改变。用WS培养HS-P细胞后，核因子κB向核内的转运增加。 WS感染大鼠脾脏和肝脏中巨噬细胞集落刺激因子（M-CSF）和粒细胞巨噬细胞集落刺激因子mRNA水平高于未感染大鼠，表明WS促进了巨噬细胞增殖。与WS共培养的HS-P细胞中IL-12p40 mRNA的水平随着针对M-CSF的小干扰RNA的转染或添加抗M-CSF抗体而增加。这些结果表明，WS通过促进巨噬细胞产生巨噬细胞集落刺激因子而抑制IL-12p40 mRNA的产生。

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