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首页> 外文期刊>Journal of Parasitology >EVALUATION OF TWO TOXOPLASMA GONDII SEROLOGIC TESTS USED IN A SEROSURVEY OF DOMESTIC CATS IN CALIFORNIA
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EVALUATION OF TWO TOXOPLASMA GONDII SEROLOGIC TESTS USED IN A SEROSURVEY OF DOMESTIC CATS IN CALIFORNIA

机译:加利福尼亚州家猫血清学检查中使用的两种弓形虫血清学测试的评价

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We evaluated the sensitivity (Se) and specificity (Sp) of an IgG enzyme-linked immunosorbent assay (ELISA) and IgG indirect fluorescent antibody test (IFAT) for detection of Toxoplasma gondii-specific antibodies in sera from 2 cat populations using a Bayesian approach. Accounting for test covariance, the Se and Sp of the IgG ELISA were estimated to be 92.6% and 96.5%, and those of the IgG IFAT were 81.0% and 93.8%, respectively. Both tests performed poorly in cats experimentally co-infected with feline immunodeficiency virus and T. gondii. Excluding this group, Se and Sp of the ELISA were virtually unchanged (92.3% and 96.4%, respectively), whereas the IFAT Se improved to 94.2% and Sp remained stable at 93.7%. These tests and an IgM ELISA were applied to 123 cat sera from the Morro Bay area, California, where high morbidity and mortality attributable to toxoplasmosis have been detected in southern sea otters. Age-adjusted IgG seroprevalence in this population was estimated to be 29.6%, and it did not differ between owned and unowned cats. Accounting for Se, Sp, and test covariances, age-adjusted seroprevalence was 45.0%. The odds for T. gondii seropositivity were 12.3-fold higher for cats aged >12 mo compared with cats aged <6 mo.
机译:我们使用贝叶斯方法评估了IgG酶联免疫吸附测定(ELISA)和IgG间接荧光抗体试验(IFAT)的灵敏度(Se)和特异性(Sp),用于检测2猫群中血清中的弓形虫特异性抗体。考虑到测试的协方差,估计IgG ELISA的Se和Sp分别为92.6%和96.5%,而IgG IFAT的Se和Sp分别为81.0%和93.8%。在实验中与猫免疫缺陷病毒和弓形虫共感染的猫中,两种测试的效果均较差。除此组外,ELISA的Se和Sp几乎没有变化(分别为92.3%和96.4%),而IFAT Se提高到94.2%,Sp保持稳定在93.7%。这些测试和IgM ELISA被应用于加利福尼亚州莫罗湾地区的123只猫血清,在南部海獭中发现了弓形虫病的高发病率和高死亡率。在该人群中,经年龄调整后的IgG血清阳性率估计为29.6%,在拥有和不拥有的猫之间没有差异。考虑到Se,Sp和检验协方差,年龄校正后的血清阳性率为45.0%。年龄大于12个月的猫的刚地弓形虫血清阳性的几率比年龄小于6个月的猫高12.3倍。

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