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首页> 外文期刊>Journal of Parasitology >ENCYSTMENT AND METACERCARIAE DEVELOPMENT OF ECHINOSTOMA CINETORCHIS CERCARIAE IN AN IN VITRO CULTURE SYSTEM
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ENCYSTMENT AND METACERCARIAE DEVELOPMENT OF ECHINOSTOMA CINETORCHIS CERCARIAE IN AN IN VITRO CULTURE SYSTEM

机译：体外培养系统中棘皮CHI虫的诱变和代谢

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摘要

For some species of 37-collar-spined Echinostoma, their cercariae successfully encyst and develop to metacercariae in vitro. In our study, we cultured Echinostoma cinetorchis cercariae in 12 different media to study the formation of metacercariae. Locke's solution, medium 199, and RPMI 1640 were used as media for culture. RPMI 1640 produced the highest encystment and normal metacercariae development. The osmolality of the media was related to their ability to encyst and develop. The 0.5× media induced higher encystment and normal metacercaria formation than the 1× media. The addition of fetal bovine serum to RPMI 1640 increased the level of encystment and normal metacercariae development. In the mixture of 0.5× RPMI 1640 and 10% fetal bovine serum, encystment was highest, at 96.0%, and the development ratio of normal metacercariae was also the highest, at 91.5%, after 48 hr of cultivation. In a viability test, 7 day- and 14 day-cultured metacercariae were successfully matured to adult worms in experimentally infected rats. These results showed that E. cinetorchis cercariae could be cultured to viable metacercariae in an in vitro culture system and that 0.5× RPMI 1640 plus 10% fetal bovine serum was the most useful medium for cultivation. This culture system can be adapted for additional studies on the E. cinetorchis life cycle, especially to supply large numbers of metacercariae for other studies on this echinostome.
机译：对于某些37轴旋转棘突棘皮瘤，它们的尾c成功地被包囊并在体外发育成尾meta。在我们的研究中，我们在12种不同的培养基中培养了灰棘棘chin鱼（Echinostoma cinetorchis cercariae），以研究meta虫的形成。洛克的溶液，培养基199和RPMI 1640被用作培养的培养基。 RPMI 1640产生最高的囊肿和正常的cer尾虫发育。介质的重量克分子渗透压浓度与其包囊和发育的能力有关。 0.5x培养基比1x培养基诱导更高的囊肿和正常的尾cer形成。将胎牛血清添加到RPMI 1640中可增加包囊水平和正常的cer尾development发育。在0.5×RPMI 1640和10％胎牛血清的混合物中，培养48小时后，囊肿率最高，为96.0％，正常normal尾虫的发育率也最高，为91.5％。在一项生存力测试中，经过7天和14天培养的尾cer在实验感染的大鼠中成功地成熟为成虫。这些结果表明，在体外培养系统中可以将灰尾大肠杆菌（E.cinetorchis cercariae）培养成可行的尾cer，而0.5×RPMI 1640加10％胎牛血清是最有用的培养基。该培养系统可适用于有关灰棘大肠杆菌生命周期的其他研究，尤其是为该棘皮动物的其他研究提供大量的尾cer。

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