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首页> 外文期刊>Journal of Parasitology >BLOOD PARASITES OF CHICKENS IN UGANDA AND CAMEROON WITH MOLECULAR DESCRIPTIONS OF LEUCOCYTOZOON SCHOUTEDENI AND TRYPANOSOMA GALLINARUM
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BLOOD PARASITES OF CHICKENS IN UGANDA AND CAMEROON WITH MOLECULAR DESCRIPTIONS OF LEUCOCYTOZOON SCHOUTEDENI AND TRYPANOSOMA GALLINARUM

机译：乌干达和喀麦隆的鸡血寄生虫的分子描述为Schuchedeni和Trypanosoma gallinarum的分子描述

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摘要

Using microscopy and PCR, we determined the prevalence of blood parasites in village chickens in Uganda and Cameroon. Of 148 individuals tested, 18.3% were infected with Leucocytozoon schoutedeni (Haemosporida, Leucocytozoidae) and 4.1% were infected with Trypanosoma gallinarum (Kinetoplastida, Trypanosomatidae). No other blood parasites were detected. Subsequent phylogenetic analysis of the cytochrome b gene of L. schoutedeni identified 2 distinct lineages that were found at all 3 sampling locations in Uganda. The sequence divergence between these 2 lineages is 1.5%. One of these lineages was also found in chickens in Cameroon, nearly 2,000 km distant. There are no morphological differences between blood stages of the parasites represented by the 2 different lineages, suggesting that cytochrome b gene sequence divergence can be as high as 1.5% within a single well-defined morphospecies of Leucocytozoon. We sequenced a portion of the small subunit ribosomal RNA gene (SSU rRNA) of T. gallinarum, and redescribe T. gallinarum for the first time since its discovery in 1911. These are the first assignments of DNA sequence data to these morphospecies of Leucocytozoon and Trypanosoma and may represent an example of intraspecific sequence divergence.
机译：使用显微镜和PCR，我们确定了乌干达和喀麦隆乡村鸡中血液寄生虫的患病率。在测试的148个个体中，有18.3％的人感染了Scheuedeni白细胞假单胞菌（Haemosporida，Leucocytozoidae），而4.1％的人感染了鸡锥虫（Kinetoplastida，Trypanosomatidae）。未检测到其他血液寄生虫。随后对L. schoutedeni的细胞色素b基因进行系统发育分析，确定了在乌干达的所有3个采样点均发现的2个不同谱系。这两个谱系之间的序列差异为1.5％。在距离喀麦隆近2,000公里的鸡群中也发现了这些血统之一。在这两个不同谱系所代表的寄生虫的血液阶段之间没有形态学差异，这表明在一个明确定义的白细胞动物形态学物种内，细胞色素b基因序列的差异可能高达1.5％。我们对T. gallinarum小亚基核糖体RNA基因（SSU rRNA）的一部分进行了测序，并自1911年发现以来首次重新描述了T. gallinarum。这是对这些Leucocytozoon和锥虫瘤可能代表种内序列差异的一个例子。

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