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首页> 外文期刊>Journal of Parasitology >FUNCTIONAL CHARACTERIZATION OF THE SERINE/THREONINE PROTEIN PHOSPHATASE 5 FROM TRYPANOSOMA BRUCEI
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FUNCTIONAL CHARACTERIZATION OF THE SERINE/THREONINE PROTEIN PHOSPHATASE 5 FROM TRYPANOSOMA BRUCEI

机译：布鲁氏锥虫中丝氨酸/苏氨酸蛋白磷酸酶5的功能鉴定

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摘要

PP5 is a member of the PPP family of serine/threonine protein phosphatases and is present in all eukaryotes. We previously cloned and characterized a PP5 homologue from Trypanosoma brucei. Here, we synchronized the T. brucei procyclic form by hydroxyurea treatment and showed that TbPP5 expression is regulated during cell cycle progression. TbPP5 transcript and protein levels were maximal in the G1 phase of the cell cycle, and reduced about 3-fold in the G2/M phase. To further evaluate its function, TbPP5 expression was depleted in both procyclic and bloodstream forms of T. brucei by RNA interference. In the procyclic form, TbPP5 knockdown resulted in a moderate reduction in cell growth. However, in the bloodstream form, ablation of TbPP5 caused an 8-fold decrease in cell growth. Furthermore, TbPP5 overexpression conferred the ability of procyclic cells to grow in serum-deprived conditions suggesting that TbPP5 acts downstream of serum factor–induced growth in T. brucei. Taken together; these findings suggest that a serum factor (or factors) induces up-regulation of TbPP5 expression during the G1 phase, which is required for proper cell growth.
机译：PP5是丝氨酸/苏氨酸蛋白磷酸酶PPP家族的成员，并且存在于所有真核生物中。我们以前克隆和表征了布鲁氏锥虫的PP5同源物。在这里，我们通过羟基脲处理同步了布鲁氏菌的前环形式，并表明在细胞周期进程中TbPP5表达受到调控。 TbPP5转录本和蛋白质水平在细胞周期的G1期达到最高，而在G2 / M期降低约3倍。为了进一步评估其功能，TbPP5的表达通过RNA干扰在布鲁氏菌的前环和血流形式中均被消耗。在前环形式中，TbPP5敲低导致细胞生长适度减少。但是，在血液中，TbPP5的消融导致细胞生长降低8倍。此外，TbPP5的过表达赋予了前列腺癌细胞在血清缺乏的条件下生长的能力，这表明TbPP5在血清因子诱导的布鲁氏菌生长中下游起作用。一起这些发现表明，血清因子（或多种因子）可诱导G1期TbPP5表达的上调，这是正常细胞生长所必需的。

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