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首页> 外文期刊>Journal of Parasitology >NEOSPORA CANINUM–LIKE OOCYSTS OBSERVED IN FECES OF FREE-RANGING RED FOXES (VULPES VULPES) AND COYOTES (CANIS LATRANS)
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NEOSPORA CANINUM–LIKE OOCYSTS OBSERVED IN FECES OF FREE-RANGING RED FOXES (VULPES VULPES) AND COYOTES (CANIS LATRANS)

机译：在自由散养的红狐狸（狐狸狐狸）和郊狼（卡尼斯拉提斯犬）的粪便中观察到象新孢子虫一样的卵囊

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摘要

The aim of this study was to examine the feces of free-ranging foxes and coyotes for the presence of Neospora caninum oocysts. Feces were collected from 271 foxes and 185 coyotes in the Canadian province of Prince Edward Island, processed by sucrose flotation, and examined by light microscopy for the presence of coccidian oocysts. In 2 fox and 2 coyote samples, oocysts morphologically and morphometrically similar to oocysts of N. caninum were observed. DNA was extracted from these samples and subjected to nested polymerase chain reaction (PCR) using primers to the N. caninum–specific Nc5 genomic sequence. Through DNA sequencing, alignment of the sequences of at least 3 clones from each isolate to sequences deposited in GenBank revealed 95–99% similarity to the Nc5 sequence of N. caninum. PCR using primers specific for Hammondia heydorni failed to yield an amplification product from these DNA samples.
机译：这项研究的目的是检查散养的狐狸和土狼的粪便中是否存在新孢子虫卵囊。从加拿大爱德华王子岛省的271只狐狸和185只土狼收集粪便，通过蔗糖浮选进行处理，并通过光学显微镜检查是否存在球虫卵囊。在2个狐狸和2个土狼样本中，观察到卵囊在形态和形态上与犬新孢子虫的卵囊相似。从这些样品中提取DNA，并使用犬新孢子虫特异性Nc5基因组序列的引物进行巢式聚合酶链反应（PCR）。通过DNA测序，将每个分离物中至少3个克隆的序列与GenBank中存放的序列进行比对，发现与犬新孢子虫的Nc5序列有95–99％的相似性。使用特异于Hammondia heydorni的引物进行PCR不能从这些DNA样品中产生扩增产物。

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