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首页> 外文期刊>Journal of Parasitology >EVALUATION OF PROCEDURES TO DETERMINE ABSOLUTE DENSITY OF PLASMODIUM VIVAX OOKINETES
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EVALUATION OF PROCEDURES TO DETERMINE ABSOLUTE DENSITY OF PLASMODIUM VIVAX OOKINETES

机译：确定疟原虫卵母细胞绝对密度的程序评价

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摘要

The ookinete is the key determinant of infection within the mosquito vector, yet there are few population studies of ookinetes in nature. This investigation compared different techniques used to estimate ookinete densities in mosquitoes. Laboratory-reared Anopheles dirus mosquitoes were fed on gametocytemic blood drawn from 7 Plasmodium vivax patients at a malaria clinic in Mae Sot, Thailand. At 20–26 hr, bloodmeals were excised. Three techniques were evaluated, i.e., hemacytometer counts under phase-contrast microscope, Giemsa staining of bloodmeal smears, and immunofluorescent staining with a monoclonal antibody specific against the 25-kDa antigen expressed on the surface of P. vivax zygotes and ookinetes. Additional mosquitoes were dissected at day 10 for oocysts. The hemacytometer method was the simplest and quickest method but lacked precision at low ookinete densities. Immunofluorescent staining was the most sensitive, accurate, and the only method that enabled unequivocal detection of zygotes. Bloodmeals contained a mixture of zygotes, retorts, and mature ookinetes, indicating that postzygotic development of P. vivax in A. dirus was asynchronous. The conversion efficiency of zygotes/ookinetes to oocysts varied among patients and was independent of zygote-ookinete density, suggesting that variations in host blood composition, e.g., antibodies, drugs, etc., may influence the success of zygote-ookinete development.
机译：蚊子是蚊媒中感染的关键决定因素，但自然界对蚊子的种群研究很少。这项调查比较了用于估计蚊子中的动子密度的不同技术。在泰国Mae Sot的一家疟疾诊所，从7名间日疟原虫患者身上抽取了配子体细胞血液，喂养了实验室饲养的按蚊di蚊。在20-26时，切掉血粉。评估了三种技术，即相差显微镜下的血细胞计数，血粉涂片的Giemsa染色以及对间日疟原虫合子和卵形钩子表面表达的25 kDa抗原具有特异性的单克隆抗体的免疫荧光染色。在第10天解剖另外的蚊子用于卵囊。血细胞计数法是最简单，最快的方法，但在低速动密度下缺乏精度。免疫荧光染色是最灵敏，准确，也是唯一能够明确检测受精卵的方法。血粉包含受精卵，ts和成熟的动植物的混合物，这表明在拟南芥中间日疟原虫的合子发育是异步的。受精卵/受精卵向卵囊的转化效率在患者之间有所不同，并且与受精卵-受精卵的密度无关，这表明宿主血液组成（例如抗体，药物等）的变化可能会影响受精卵-受精卵的发育。

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