SEQUENCE VARIATION OF THE RIBOSOMAL DNA SECOND INTERNAL TRANSCRIBED SPACER REGION IN TWO SPATIALLY DISTINCT POPULATIONS OF AMBLYOMMA AMERICANUM (L.) (ACARI: IXODIDAE)

M. V. Reichard A. A. Kocan R. A. Van Den Bussche* R. W. Barker† J. H. Wyckoff III and S. A. Ewing

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SEQUENCE VARIATION OF THE RIBOSOMAL DNA SECOND INTERNAL TRANSCRIBED SPACER REGION IN TWO SPATIALLY DISTINCT POPULATIONS OF AMBLYOMMA AMERICANUM (L.) (ACARI: IXODIDAE)

机译：美洲双歧杆菌（L.）的两个空间不同种群中核糖体DNA第二个内部翻译间隔区的序列变异（ACARI：IXODIDAE）

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Sequence analysis of the ribosomal DNA second internal transcribed spacer (ITS 2) region in 2 spatially distinct populations of Amblyomma americanum (L.) revealed intraspecific variation. Nucleotide sequences from multiple DNA extractions and several polymerase chain reaction amplifications of eggs from mixed-parentage samples from both populations of ticks revealed that 12 of 1,145 (1.0%) sites varied. Three of the 12 sites of variation were distinct between the 2 A. americanum populations, which corresponded to a rate of 0.26%. Phylogenetic analysis based on ITS 2 sequences provided strong support (i.e., bootstrap value of 80%) that wild A. americanum clustered into a distinguishable group separate from those derived from colony ticks.

机译：2个在空间上不同的美洲草（L.）种群中的核糖体DNA第二个内部转录间隔区（ITS 2）区域的序列分析揭示了种内变异。来自两个壁虱种群的混合亲本样本中鸡蛋的多次DNA提取和几个聚合酶链反应扩增的核苷酸序列显示，在1,145个（1.0％）位点中有12个发生了变化。 12个变异位点中的三个在两个美洲曲霉种群之间是不同的，对应率为0.26％。基于ITS 2序列的系统发育分析提供了有力的支持（即自举值为80％），即野生美洲A.

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《Journal of Parasitology》 |2005年第2期|p.00000260-00000263|共4页
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M. V. Reichard A. A. Kocan R. A. Van Den Bussche* R. W. Barker† J. H. Wyckoff III and S. A. Ewing;
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Department of Veterinary Pathobiology, College of Veterinary Medicine, Oklahoma State University, Stillwater, Oklahoma 74078. rmason@okstate.edu*Department of Zoology and Collection of Vertebrates, Oklahoma State University, Stillwater, Oklahoma 74078†Department of Entomology and Plant Pathology, Oklahoma State University, Stillwater, Oklahoma 74078;

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SEQUENCE VARIATION OF THE RIBOSOMAL DNA SECOND INTERNAL TRANSCRIBED SPACER REGION IN TWO SPATIALLY DISTINCT POPULATIONS OF AMBLYOMMA AMERICANUM (L.) (ACARI: IXODIDAE)

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