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首页> 外文期刊>Journal of Parasitology >INFLUENCE OF PHOSPHOLIPID COMPOSITION ON THE ADJUVANTICITY AND PROTECTIVE EFFICACY OF LIPOSOME-ENCAPSULATED LEISHMANIA DONOVANI ANTIGENS
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INFLUENCE OF PHOSPHOLIPID COMPOSITION ON THE ADJUVANTICITY AND PROTECTIVE EFFICACY OF LIPOSOME-ENCAPSULATED LEISHMANIA DONOVANI ANTIGENS

机译：磷脂组成对脂质体包裹的利什曼原虫DONOVANI抗原的耐受性和保护功效的影响

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摘要

In this study, we evaluate the effect of phospholipid on the adjuvanicity and protective efficacy of liposome vaccine carriers against visceral leishmaniasis (VL) in a hamster model. Liposomes prepared with distearyol derivative of l-α-phosphatidyl choline (DSPC) having liquid crystalline transition temperature (Tc) 54 C were as efficient as dipalmitoyl (DPPC) (Tc 41 C) and dimyristoyl (DMPC) (Tc 23 C) derivatives in their ability to entrap Leishmania donovani membrane antigens (LAg) and to potentiate strong antigen-specific antibody responses. However, whereas LAg in DPPC and DMPC liposomes stimulated inconsistent delayed type hypersensitivity (DTH) responses, strong DTH was observed with LAg in DSPC liposomes. The heightened adjuvant activity of DSPC liposomes corresponded with 95% protection, with almost no protectivity with LAg in DPPC and DMPC liposomes, 4 mo after challenge with L. donovani. These data demonstrate the superiority of DSPC liposomes for formulation of L. donovani vaccine. In addition, they demonstrate a correlation of humoral and cell-mediated immunity with protection against VL in hamsters.
机译：在这项研究中，我们评估了磷脂对仓鼠模型中脂质体疫苗载体对内脏利什曼病（VL）的辅助作用和保护功效。用具有液晶转变温度（Tc）54°C的l-α-磷脂酰胆碱（DSPC）的二芳醇衍生物制备的脂质体与二棕榈油酰基（DPPC）（Tc 41 C）和二肉豆蔻酰基（DMPC）（Tc 23 C）衍生物的效率相同它们具有捕获多形利什曼原虫膜抗原（LAg）和增强强烈的抗原特异性抗体反应的能力。然而，虽然DPPC和DMPC脂质体中的LAg刺激了不一致的延迟型超敏反应（DTH）反应，但在DSPC脂质体中LAg观察到了强DTH。 DSPC脂质体的佐剂活性提高对应于95％的保护作用，在用多诺尼乳杆菌攻击4个月后，对DPPC和DMPC脂质体中的LAg几乎没有保护作用。这些数据证明了DSPC脂质体对多诺氏乳杆菌疫苗的配制的优越性。此外，他们证明了体液和细胞介导的免疫力与仓鼠的VL保护作用相关。

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    《Journal of Parasitology》 |2005年第2期|p.00000269-00000274|共6页
  • 作者

    Tuhina Mazumdar* K. Anam† N. Ali‡;

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    Infectious Diseases Group, Indian Institute of Chemical Biology, 4, Raja S. C. Mullick Road, Kolkata 700032, India. nali@iicb.res.in*Present address: Department of Medicine, Pulmonary Division, Baylor College of Medicine, One Baylor Plaza, Houston, Texas 77030†WIDDK, Navy Transplantation Autoimmunity Branch, National Institutes of Health, AFRRI Building, Room 2416 B, 8901 Wisconsin Ave, Bethesda, Maryland 20889‡To whom correspondence should be addressed;

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