...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Parasitology >Polymerase Chain Reaction Detection of Cytauxzoon felis From Field-Collected Ticks and Sequence Analysis of the Small Subunit and Internal Transcribed Spacer 1 Region of the Ribosomal RNA Gene
【24h】

Polymerase Chain Reaction Detection of Cytauxzoon felis From Field-Collected Ticks and Sequence Analysis of the Small Subunit and Internal Transcribed Spacer 1 Region of the Ribosomal RNA Gene

机译：从现场采集的Ti虫中提取出的Cy虫的聚合酶链反应以及核糖体RNA基因小亚基和内部转录间隔区1区域的序列分析

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Cytauxzoon felis produces a disease in domestic cats in the Midwest (U.S.A.), which often leads to a fatal outcome. Although the clinical disease process is well described, there are still many unanswered questions about this organism. For example, it is unknown whether species of ticks other than Dermacentor variabilis can serve as vectors for transmission. With recent reports of surviving cats from limited geographic areas, another relevant question is the potential for genetically less virulent organism strains. This study evaluated 352 individual or pooled tick samples (1,362 total ticks) for the presence of C. felis small subunit ribosomal RNA and internal transcribed spacer 1 (ITS-1) region genes using a polymerase chain reaction (PCR). These ticks were collected from dogs and cats in several Missouri counties, including 10 from cats diagnosed with cytauxzoonosis. Only 3 positive C. felis samples were identified in Amblyomma americanum nymphs, and there was very limited genetic variation noted in both genes. The small number of positive samples did not allow the study to determine which PCR analysis was more sensitive. This is the first known report of ITS-1 gene identification and sequencing for C. felis. It is also the first published investigation of genetic variation in C. felis.
机译：Cytauxzoon felis在中西部（美国）的家猫中产生一种疾病，通常会导致致命的后果。尽管已经很好地描述了临床疾病过程，但是关于这种生物仍然有许多未解决的问题。例如，不知道除变种真皮外的其他壁虱是否可以作为传播的载体。最近有报道说猫只在有限的地理区域内生存，另一个相关的问题是遗传上毒性较低的生物菌株的潜力。这项研究使用聚合酶链反应（PCR）评估了352个单独或合并的壁虱样本（总共1,362个壁虱）中是否存在猫屎肠杆菌小亚基核糖体RNA和内部转录间隔区1（ITS-1）基因。这些壁虱是从密苏里州的几个县的猫和狗身上采集的，其中10个是从被诊断为囊性动物病的猫身上采集的。在美洲盲mb若虫中仅鉴定出3个阳性的猫隐球菌样品，并且在两个基因中都注意到非常有限的遗传变异。少量阳性样品无法使研究确定哪种PCR分析更敏感。这是第一份已知的ITS-1基因鉴定和猫屎链球菌测序报告。这也是首次公开发表的关于猫屎球菌遗传变异的研究。

著录项

  • 来源
    《Journal of Parasitology》 |2005年第2期|p.00000458-00000461|共4页
  • 作者

    P. J. Bondy Jr.a L. A. Cohna J. W. Tylera and A. E. Marsh*†abc;

  • 作者单位

    aDepartment of Veterinary Medicine and Surgery, College of Veterinary Medicine, University of Missouri, Columbia, Missouri 65211 bDepartment of Veterinary Preventive Medicine, The Ohio State University, Columbus, Ohio 43210 cTo whom correspondence should be addressed. marsh.2061@osu.edu;

  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号