Human Malaria Parasites in Continuous Culture

William Trager and James B. Jensen

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Human Malaria Parasites in Continuous Culture

机译：连续文化中的人类疟疾寄生虫

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Plasmodium falciparum can now be maintained in continuous culture in human erythrocytes incubated at 38°C in RPMI 1640 medium with human serum under an atmosphere with 7 percent carbon dioxide and low oxygen (1 or 5 percent). The original parasite material, derived from an infected Aotus trivirgatus monkey, was diluted more than 100 million times by the addition of human erythrocytes at 3- or 4-day intervals. The parasites continued to reproduce in their normal asexual cycle of approximately 48 hours but were no longer highly synchronous. They have remained infective to Aotus.

机译：恶性疟原虫现在可以在含有人血清的RPMI 1640培养基中在38％的二氧化碳和低氧（1％或5％）的气氛下于38°C孵育的人类红细胞中连续培养。通过以3或4天的间隔添加人红细胞，将源自感染的Aotus trivirgatus猴子的原始寄生物稀释超过1亿倍。寄生虫继续以大约48小时的正常无性繁殖，但不再高度同步。他们仍然感染Aotus。

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《Journal of Parasitology》 |2005年第3期|p.00000484-00000486|共3页
作者
William Trager and James B. Jensen;
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Department of Parasitology, Rockefeller University, New York 10021;

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Human Malaria Parasites in Continuous Culture

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