...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Parasitology >Kinetics of Antibodies and Antigens in Serum of Mice Experimentally Infected with Echinostoma caproni (Trematoda: Echinostomatidae)
【24h】

Kinetics of Antibodies and Antigens in Serum of Mice Experimentally Infected with Echinostoma caproni (Trematoda: Echinostomatidae)

机译：实验性感染卡氏棘孢E虫（小鼠）的血清中抗原和抗原的动力学

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The present study reports on the kinetics of antibodies and antigens in serum of mice experimentally infected with 75 metacercariae of Echinostoma caproni during the first 12 wk postinfection (wpi). Antibody titers in the serum of mice were determined by an indirect enzyme-linked immunosorbent assay (ELISA) using excretory/secretory (ES) antigens of E. caproni. The early detection of antibodies against ES antigens of E. caproni is feasible using indirect ELISA. Mice developed significant antibody responses at 2 wpi, and the values progressively increased until the end of the experiment. This may be related to the intestinal absorption of adult worm antigens that induces humoral responses. The presence of E. caproni circulating antigens was determined by a capture ELISA based on polyclonal rabbit antibodies against ES antigens of E. caproni. High levels of seroantigens in mice were detected by 1–2 wpi, probably because of the local inflammatory responses in mice induced by the adult worms. A drop in circulating antigen levels was observed at 9 wpi, which could reflect changes in the intestinal tissues over the course of the infection.
机译：本研究报告了在感染后的前12周（wpi）期间实验性感染了75例卡氏棘孢棘球meta虫的小鼠血清中抗体和抗原的动力学。小鼠血清中的抗体效价是通过间接酶联免疫吸附测定（ELISA）使用卡普罗氏大肠杆菌的排泄/分泌（ES）抗原确定的。使用间接ELISA可以早期检测出针对caproni的ES抗原的抗体。小鼠在2 wpi时产生了显着的抗体反应，并且该值逐渐增加直至实​​验结束。这可能与诱导体液反应的成虫蠕虫抗原在肠道的吸收有关。通过基于针对caproni ES抗原的多克隆兔抗体的捕获ELISA，确定caproni循环抗原的存在。 1–2 wpi可检测到小鼠体内高水平的血清抗原，这可能是由于成虫引起的小鼠局部炎症反应。在9 wpi时观察到循环抗原水平下降，这可能反映了感染过程中肠道组织的变化。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号