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首页> 外文期刊>Journal of Parasitology >Isolation, Tissue Distribution, and Molecular Characterization of Toxoplasma gondii From Free-Range Chickens From Guatemala
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Isolation, Tissue Distribution, and Molecular Characterization of Toxoplasma gondii From Free-Range Chickens From Guatemala

机译：危地马拉散养鸡弓形虫的分离，组织分布和分子特征

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The prevalence of Toxoplasma gondii in free-ranging chickens is a good indicator of the prevalence of T. gondii oocysts in the soil because chickens feed from the ground. The prevalence of T. gondii in 50 free-range chickens (Gallus domesticus) from Guatemala was determined. Antibodies to T. gondii were assayed by the modified agglutination test (MAT). Antibodies were found in 37 (74%) chickens with titers of 1:5 (11), 1:10 (7), 1:20 (11), 1:40 (1), 1:80 (1), 1:160 (3), 1:1,280 (2), and 1:2,560 (1). Hearts, pectoral muscles, and brains of 19 chickens with MAT titers of 1:20 or more were bioassayed individually in mice. Tissues from the remaining 31 chickens with titers of 1:10 or lower were pooled and fed to 4 T. gondii–free cats (13 chickens with titers of less than 1:5 to 1 cat, 11 chickens with titers of 1:5 to 2 cats, and 7 chickens with titers of 1:10 to 1 cat). Feces of cats were examined for oocysts; they did not shed oocysts. Toxoplasma gondii was isolated from 8 chickens with MAT titers of 1:20 or more (from 1 of 11 chickens with a titer of 1:20 and all 7 chickens with a titer of 1:80 or more) from the heart, brain, and pectoral muscle (3); heart and pectoral muscle (1); and heart alone (4). Genotyping of these 8 isolates with the SAG2 locus indicated that 5 were type III and 3 were type I. This is the first report of isolation of T. gondii from chickens from Guatemala.
机译：散养鸡中的弓形虫患病率是土壤中弓形虫卵囊患病率的良好指标，因为鸡是从地面进食的。确定了来自危地马拉的50只散养鸡（家蝇）中的弓形虫患病率。通过改良的凝集试验（MAT）测定弓形虫的抗体。在37只（74％）鸡中发现了抗体，滴度为1：5（11），1：10（7），1：20（11），1：40（1），1：80（1），1： 160（3），1：1,280（2）和1：2,560（1）。分别对小鼠的MAT效价为1:20或更高的19只鸡的心脏，胸肌和大脑进行了生物测定。收集其余31只滴度为1:10或更低的鸡的组织，并喂给4只无弓形虫的猫（13只滴度小于1：5的鸡至1只猫，11只滴度为1：5至5的鸡）。 2只猫和7只鸡，滴度为1:10至1猫）。检查猫的粪便中是否有卵囊。他们没有流卵囊。从心脏，大脑和大脑中分离出8份MAT效价为1:20或更高的鸡（从11份效价为1:20的鸡中的一只和所有7份效价为1:80或更高的鸡中分离出弓形虫）。胸肌（3）;心脏和胸肌（1）;和一个人的心（4）。用SAG2基因座对这8个分离株进行基因分型表明，其中5个是III型，而3个是I型。这是从危地马拉的鸡中分离弓形虫的第一个报道。

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    《Journal of Parasitology》 |2005年第4期|p.00000955-00000957|共3页
  • 作者

    J. P. Dubeya B. Lopezb M. Alvarezb C. Mendozab and T. Lehmannc;

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    aU.S. Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Animal Parasitic Diseases Laboratory, Building 1001, Beltsville, Maryland 20705-2350 bCentro de Estudios en Salud, Universidad del Valle de Guatemala, 18 Ave. 11-35 Zona 15 VH III, Guatemala City, Guatemala cDivision of Parasitic Diseases, Centers for Disease Control and Prevention, 4770 Buford Highway, MS: F22, Chamblee, Georgia 30341. jdubey@anri.barc.usda.gov;

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