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首页> 外文期刊>Journal of Parasitology >MOLECULAR CHARACTERIZATION OF TRICHOMONADS FROM FECES OF DOGS WITH DIARRHEA
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MOLECULAR CHARACTERIZATION OF TRICHOMONADS FROM FECES OF DOGS WITH DIARRHEA

机译：从腹泻犬的粪便中滴虫的分子鉴定

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摘要

Trichomonads are occasionally observed in the feces of dogs with diarrhea. On the basis of superficial morphological appearance, these infections have been attributed to opportunistic overgrowth of the commensal, Pentatrichomonas hominis. However, molecular characterization of canine trichomonads has never been reported. This study was performed to determine, by means of rRNA gene sequence analysis, the identity of trichomonads observed in feces from dogs with diarrhea. Total DNA was isolated from fecal samples obtained from a 3-mo-old mixed breed dog and litter of German Shepherd puppies having profuse liquid diarrhea containing numerous trichomonads. Total DNA was subject to PCR amplification of partial 18S rRNA gene or 5.8S, ITS1, ITS2, and partial 18S and 28S rRNA genes using species-specific and universal primers, respectively. Products of 642 and 1864 base-pair length were amplified and cloned. On the basis of rRNA gene sequence, the trichomonads observed in the single dog and the litter of puppies shared 100% identity with Tritrichomonas foetus and P. hominis, respectively. The present study is the first to establish the molecular identity of trichomonads infecting dogs with diarrhea. These studies validate the longstanding assumption that canine trichomoniasis may be attributed to P. hominis. Importantly, these studies additionally recognize that canine trichomoniasis may also be caused by infection with T. foetus.
机译：腹泻犬的粪便中偶尔会观察到滴虫。基于表面形态学的出现，这些感染归因于共生五角形人（Pentatrichomonas hominis）的机会性过度生长。但是，尚未报道犬毛滴虫的分子表征。通过rRNA基因序列分析来进行这项研究，以确定在腹泻犬粪便中观察到的滴虫的身份。从粪便样品中分离总DNA，该粪便样品取自3个月大的混合犬和具有大量滴虫性腹泻的大量液体腹泻的德国牧羊犬幼犬的窝。分别使用物种特异性引物和通用引物对总DNA进行部分18S rRNA基因或5.8S，ITS1，ITS2以及部分18S和28S rRNA基因的PCR扩增。 642和1864个碱基对长度的产物被扩增并克隆。根据rRNA基因序列，在单犬和幼犬幼仔中观察到的毛滴虫分别与胎儿毛滴虫和人性疟原虫有100％的同一性。本研究是第一个确定滴虫感染腹泻犬的分子身份的方法。这些研究证实了长期以来的假设，即犬毛滴虫病可能归因于人型疟原虫。重要的是，这些研究还认识到犬毛滴虫病也可能是由胎儿鞭毛虫感染引起的。

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    《Journal of Parasitology》 |2005年第4期|p.00000939-00000943|共5页
  • 作者

    Jody L. Gookin Adam J. Birkenheuer* Victoria St. John Michelle Spector† and Michael G. Levy‡;

  • 作者单位

    Department of Molecular Biomedical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, North Carolina 27606. jody_gookin@ncsu.edu*Clinical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, North Carolina 27606†Fred Hutchinson Cancer Research Center, Seattle, Washington 98109‡Department of Farm Animal Health and Resource Medicine, College of Veterinary Medicine, North Carolina State University, Raleigh, North Carolina 27606;

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