• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Parasitlolgy >SCHISTOSOMA MANSONI: ANTIGEN-PRESENTING CELLS EMIGRATING FROM SKIN EXPOSED TO ATTENUATED CERCARIAE ACTIVATE LYMPHOID CELLS AND TRANSFER PROTECTION IN C57Bl/6 MICE
【24h】

SCHISTOSOMA MANSONI: ANTIGEN-PRESENTING CELLS EMIGRATING FROM SKIN EXPOSED TO ATTENUATED CERCARIAE ACTIVATE LYMPHOID CELLS AND TRANSFER PROTECTION IN C57Bl/6 MICE

机译：血吸虫血吸虫病：从皮肤暴露到减毒的尾肠活化的淋巴瘤细胞中的抗原表达细胞在C57Bl / 6小鼠中的转移保护

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

C57Bl/6 mice develop significant levels of protection to a challenge infection after percutaneous exposure to irradiated Schistosoma mansoni cercariae. Although some circumstantial evidence has suggested that antigen-presenting cells (APCs) within the skin play a role in priming anti-schistosomula effector mechanisms, no direct evidence has been presented. In this study, we describe efforts to directly test whether skin-resident APCs exposed to irradiated cercariae are capable of mediating responses consistent with previously proposed mechanisms associated with delayed-type hypersensitivity reactions. We demonstrate that a population of APCs emigrates from the skin after percutaneous vaccination and that these cells are able to induce proliferation of S. mansoni–specific lymphocytes. We describe our experiments conducted to confirm that proliferation is dependent on major histocompatibility complex (MHC) Class-II interactions and cell-to-cell contact between APCs and lymphocytes. Immunohistological staining of emigrating cells revealed a population of large MHC Class-II+ cells with a morphology characteristic of mature dendritic cells. On recovery and adoptive transfer into naive mice, these cells demonstrated the ability to mediate protection to a challenge infection at levels similar to those in percutaneously vaccinated controls. This confirms that cutaneous APCs can initiate anti–schistosomula effector mechanisms in C57Bl/6 mice after percutaneous vaccination.
机译：在经皮暴露于曼氏血吸虫尾c的经皮暴露后，C57Bl / 6小鼠对攻击性感染产生了显着水平的保护。尽管一些间接证据表明皮肤内的抗原呈递细胞（APC）在引发抗血吸虫效应子机制中起作用，但目前尚无直接证据。在这项研究中，我们描述了直接测试暴露于辐射尾cer的皮肤驻留APC是否能够介导与先前提出的与迟发型超敏反应相关的机制一致的反应的努力。我们证明，经皮注射疫苗后，APC会从皮肤移出，并且这些细胞能够诱导曼氏沙门氏菌特异性淋巴细胞的增殖。我们描述了我们进行的实验，以确认增殖依赖于主要的组织相容性复合物（MHC）II类相互作用以及APC与淋巴细胞之间的细胞间接触。迁移细胞的免疫组织学染色显示，一群大型MHC II +类细胞具有成熟的树突状细胞形态特征。在恢复和过继转移到幼稚小鼠中后，这些细胞表现出了以与经皮疫苗接种的对照相似的水平介导针对攻击性感染的保护的能力。这证实了经皮疫苗接种后，皮肤APC可以启动C57Bl / 6小鼠的抗血吸虫效应子机制。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号