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首页> 外文期刊>Journal of Parasitology >Development of Patent Infection in Immunosuppressed C57BL/6 Mice with a Single Cryptosporidium meleagridis Oocyst
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Development of Patent Infection in Immunosuppressed C57BL/6 Mice with a Single Cryptosporidium meleagridis Oocyst

机译：免疫抑制的C57BL / 6小鼠与单个隐孢子虫卵囊的专利感染的发展。

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The ability of Cryptosporidium meleagridis to produce patent infection was studied in adult C57BL/6 mice that were immunosuppressed with dexamethasone phosphate provided in the drinking water at a dosage of 16 μg/ml. Four days after the onset of immunosuppression, mice were orally challenged with 1, 3, 10, or 1,000 C. meleagridis TU1867 oocysts per mouse. The mice were monitored daily for 18 days postinoculation for oocyst shedding. Five of 10 mice given a single oocyst, 4 of 5 mice given 3 oocysts, and all 9 mice given either 10 or 1,000 oocysts became infected and began shedding oocysts 5–7 days after challenge and continued to shed oocysts until the end of the experiment on day 18 postchallenge. Approximately 107 oocysts per mouse per day were excreted, regardless of the challenge dose. Neither the noninfected, immunosuppressed nor the inoculated, nonimmunosuppressed control mice shed oocysts. The excreted oocysts were confirmed to be those of C. meleagridis by polymerase chain reaction–restriction fragment length polymorphism analysis. We show that C. meleagridis, originally classified as an avian pathogen but recently found in humans with cryptosporidiosis, can produce patent infection in mice infected with a single oocyst. Moreover, we demonstrate that the immunosuppressed C57BL/6 adult mouse is an ideal host for the propagation of clonal populations of C. meleagridis isolates for laboratory studies.
机译：在成年C57BL / 6小鼠中研究了麻疯隐孢子虫产生专利感染的能力，这些小鼠被饮用水中提供的磷酸地塞米松以16μg/ ml的剂量免疫抑制。免疫抑制开始四天后，每只小鼠口服1、3、10或1,000个C. meleagridis TU1867卵囊攻击小鼠。接种后18天每天监测小鼠卵囊脱落。 10只小鼠中的5只接受单个卵囊，5只小鼠中的4只给予3个卵囊，而所有9只接受10或1,000个卵囊的小鼠被感染并在攻击后5-7天开始脱落卵囊，并继续脱落卵囊直至实验结束在挑战赛第18天。不管激发剂量如何，每天每只小鼠大约排出107个卵囊。未感染，未免疫抑制或未接种，未免疫抑制的对照小鼠均未脱落卵囊。通过聚合酶链反应-限制性片段长度多态性分析，证实排出的卵囊是米氏梭菌。我们显示C. meleagridis，最初被分类为禽病原体，但最近在隐孢子虫病的人类中发现，可以在感染单个卵囊的小鼠中产生专利感染。此外，我们证明了免疫抑制的C57BL / 6成年小鼠是理想的宿主，可用于实验室研究的meleagridis分离株克隆种群的繁殖。

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    《Journal of Parasitology》 |2003年第3期|p.620-622|共3页
  • 作者

    Kehe Huanga Donna E. Akiyoshibc Xiaochuan Fengb and Saul Tziporib;

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    aCollege of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, Jiangsu Province, People's Republic of China bDepartment of Biomedical Sciences, Tufts University School of Veterinary Medicine, North Grafton, Massachusetts 01536 cTo whom correspondence should be addressed. donna.akiyoshi@tufts.edu;

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