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Preliminary Molecular Characterization of Cryptosporidium parvum Isolates of Wildlife Rodents From Poland

机译：波兰野生啮齿动物隐孢子虫分离株的初步分子表征

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摘要

Isolates of Cryptosporidium were collected from 3 species of woodland and field rodents (Clethrionomys glareolus, Microtus arvalis, and Apodemus flavicollis) and were characterized by polymerase chain reaction amplification and sequencing of fragments of the oocyst wall protein (COWP) gene and of the 18S ribosomal RNA gene. Sequence analysis of these markers revealed that the animals were infected with C. parvum, and that the genotype involved was almost identical to the mouse genotype previously described from Mus musculus. Thus, small rodents should be considered as an important reservoir of C. parvum genotypes closely related to the zoonotic genotype 2 and potentially hazardous to humans.
机译：隐孢子虫的分离物从3种林地和野地啮齿类动物（食蟹鼠（Clethrionomys glareolus），田鼠（Microtus arvalis）和黄线姬鼠（Apodemus flavicollis））收集，其特征在于聚合酶链反应扩增以及卵囊壁蛋白（COWP）基因和18S核糖体片段的测序RNA基因。这些标记物的序列分析表明，动物感染了小球藻，并且涉及的基因型与先前从小家鼠中描述的小鼠基因型几乎相同。因此，小型啮齿类动物应被视为与人畜共患病基因型2密切相关并可能对人类构成危险的小小隐孢子虫基因型的重要储存库。

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  • 来源
    《Journal of Parasitology》 |2003年第5期|p.1053-1055|共3页
  • 作者

    A. Bajera S. Cacciòb M. Bednarskaa J. M. Behnkec N. J. Pieniazekd and E. Sinskia;

  • 作者单位

    aDepartment of Parasitology, Faculty of Biology, Warsaw University, Warszawa, Miecznikowa 1, 02-096 Warsaw, Poland bLaboratory of Parasitology, Istituto Superiore di Sanità, Rome, Italy cSchool of Life & Environmental Sciences, University of Nottingham, Nottingham NG7 2RD, U.K. dDivision of Parasitic Diseases, National Center for Infectious Diseases, Centers for Diseases Control and Prevention, U.S. Department of Health and Human Services, Atlanta, Georgia. esinski@biol.uw.edu.pl;

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