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首页> 外文期刊>Journal of Parasitology >INHIBITION OF EXCYSTATION AND METACYSTIC DEVELOPMENT OF ENTAMOEBA INVADENS BY THE DINITROANILINE HERBICIDE ORYZALIN
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INHIBITION OF EXCYSTATION AND METACYSTIC DEVELOPMENT OF ENTAMOEBA INVADENS BY THE DINITROANILINE HERBICIDE ORYZALIN

机译：二硝基苯胺类除草剂Oryzalin抑制Entamoeba入侵分子的代谢和发育

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摘要

The effect of oryzalin on excystation and metacystic development of Entamoeba invadens strain IP-1 was examined by transfer of cysts to a growth medium containing the drug. Excystation, which was assessed by counting the number of metacystic amoebae after induction of excystation, was inhibited by oryzalin in a concentration-dependent manner. Metacystic development, which was determined by the number of nuclei in metacystic amoebae, was also inhibited by oryzalin because the percentage of 4-nucleate amoebae at day 1 remained unchanged at day 3. The addition of oryzalin after the induction of excystation decreased the number of metacystic amoebae, compared with control cultures. When cysts were incubated for 1 day in growth medium plus oryzalin, little increase in the number of metacystic amoebae was observed after removal of the drug. Excystation and metacystic development were further inhibited when the cysts were incubated for 30 min in encystation medium containing oryzalin before transfer to growth medium with the drug. When cysts were incubated for 30 min in encystation medium before transfer to growth medium without the drug, metacystic amoebae decreased in number. Pretreatment of cysts with oryzalin for 30 min in phosphate-buffered saline markedly reduced viability and prevented excystation in growth medium with or without the drug. The results indicate that oryzalin inhibits excystation and metacystic development of E. invadens, suggesting that it may be an inhibitor of Entamoeba infection.
机译：通过将囊肿转移到含有该药物的生长培养基中来检查稻草素对侵染Entamooeba invadens菌株IP-1的兴奋性和囊胚发育的作用。谷胱甘肽以浓度依赖性的方式抑制了囊胚形成，囊胚形成是通过计数诱导诱发兴奋后的囊性变形虫数量来评估的。由于在第1天第4天第4核变形虫的百分率在第3天仍保持不变，因此，谷胱甘肽也抑制了由囊状变形虫中核数决定的囊胚发育，因为第1天4核变形虫的百分比保持不变。囊性变形虫，与对照培养相比。当囊肿在生长培养基中加米草素孵育1天时，除去药物后，观察到的囊性变形虫数量几乎没有增加。当将囊肿在含有稻谷素的浸润培养基中孵育30分钟后再转移到含有药物的生长培养基中时，囊肿和囊胚的发育进一步受到抑制。当囊肿在入侵培养基中孵育30分钟，然后转移到不含药物的生长培养基中时，后囊性变形虫数量减少。在磷酸缓冲盐溶液中用稻谷素对囊肿进行30分钟的预处理显着降低了生存能力，并防止了在有或没有药物的生长培养基中发生兴奋。结果表明，稻草素抑制入侵的大肠杆菌和侵袭性囊胚的发展，这表明它可能是Entamoeba感染的抑制剂。

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