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Rotifers Ingest Giardia Cysts

机译：轮虫摄食贾第虫囊肿

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摘要

Seven species of rotifers representing 6 genera, Epiphanes, Plationus, Asplanchna, Philodina species A, Philodina species B, Platyias, and Brachionus, were exposed to Giardia cysts isolated from the feces of experimentally infected holstein calves. Giardia cysts were prestained with a fluorescein isothiocyanate–conjugated monoclonal antibody and mixed with viable rotifers on 3-well Teflon-coated microscope slides. Organisms were observed with phase-contrast, differential interference contrast, and fluorescence microscopy. Five rotifer species, Epiphanes brachionus, Plationus patulus, Philodina (both A and B), and Platyias quadricornis, ingested varying numbers of cysts, which were retained within the rotifers' bodies throughout the observation period. Rotifer ingestion of Giardia cysts may represent a means of reducing water contamination.
机译：代表6个属的7种轮虫，即Epiphanes，Plateus，Asplanchna，Philodina种A，Philodina种B，Plattias和Brachionus，暴露于从实验感染的荷斯坦牛犊的粪便中分离出的Giardia囊肿。贾第鞭毛虫囊肿用异硫氰酸荧光素偶联的单克隆抗体进行预染色，并与有活力的轮虫混合在涂有特氟龙的3孔显微镜载玻片上。通过相差，微分干涉对比和荧光显微镜观察生物。五种轮虫种类，即Epiphanes brachionus，Plateus patulus，Philodina（A和B）和Platyias quadricornis摄取了不同数量的囊肿，这些囊肿在整个观察期内都保留在轮虫体内。轮虫摄食贾第虫囊肿可能是减少水污染的一种手段。

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  • 来源
    《Journal of Parasitology》 |2002年第5期|p.1038-1040|共3页
  • 作者

    J. M. Trouta E. J. Walshb R. Fayera;

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    aAnimal Waste Pathogen Laboratory, Animal and Natural Resources Institute, Agricultural Research Service, U.S. Department of Agriculture, Beltsville, Maryland 20705. jtrout@anri.barc.usda.gov bUniversity of Texas El Paso, Department of Biology, El Paso, Texas;

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