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首页> 外文期刊>Journal of Parasitology >Identification of Fatty Acid Molecules in a Fasciola hepatica Immunoprophylactic Fatty Acid-Binding Protein
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Identification of Fatty Acid Molecules in a Fasciola hepatica Immunoprophylactic Fatty Acid-Binding Protein

机译：肝Fasciola免疫预防性脂肪酸结合蛋白中的脂肪酸分子的鉴定

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Fasciola hepatica adult flukes have a native protein complex denoted nFh12 and consisting of fatty acid binding proteins that comprise at least 8 isoforms. It is a potent immunogen because in several animal hosts it induces an early antibody response to F. hepatica infection. It is also a potent cross-protective immunogen because it induces a protective immune response in mice to challenge infection with Schistosoma mansoni cercariae. The gene encoding this protein has been cloned and sequenced. It produces a polypeptide of 132 amino acids with a predicted molecular mass of 14.7 kDa and is denoted rFh15. It also has a significant homology to a 14-kDa S. mansoni fatty acid binding protein (Sm14). In the present study, nFh12 was delipidated with charcoal treatment and then studied by sodium dodecyl sulfate–polyacrylamide gel electrophoresis. Additionally, a lipid analysis of nFh12 was undertaken using gas chromatography–mass spectrometry to demonstrate that the nFh12 protein complex is, in fact, a complex of fatty acid binding proteins. Five long-chain saturated and unsaturated fatty acids were detected. The most abundant were palmitic acid (38%), stearic acid (24%), and oleic acid (13%). These fatty acid molecules do not have covalent bonds attached to the protein molecule. Because both nFh12 and Sm14 protect mice against challenge infection with F. hepatica and S. mansoni, it is possible that they have common protective epitopes in which fatty acids could be involved. Further studies are in progress to determine the chemical nature of these potential common epitopes.
机译：肝片吸虫（Fasciola hepatica）成年吸虫具有一个天然蛋白复合物，称为nFh12，由包含至少8个同工型的脂肪酸结合蛋白组成。它是一种有效的免疫原，因为它在数只动物宿主中诱导了对肝炎链球菌感染的早期抗体反应。它也是有效的交叉保护性免疫原，因为它在小鼠中诱导了保护性免疫应答，以挑战曼氏血吸虫尾c的感染。编码该蛋白质的基因已被克隆和测序。它产生具有132个氨基酸的多肽，预测的分子量为14.7 kDa，称为rFh15。它也与14 kDa曼氏葡萄球菌脂肪酸结合蛋白（Sm14）具有显着同源性。在本研究中，nFh12用木炭处理脱脂，然后通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳进行研究。此外，使用气相色谱-质谱法对nFh12进行了脂质分析，以证明nFh12蛋白复合物实际上是脂肪酸结合蛋白的复合物。检测到五种长链饱和和不饱和脂肪酸。最丰富的是棕榈酸（38％），硬脂酸（24％）和油酸（13％）。这些脂肪酸分子没有与蛋白质分子连接的共价键。因为nFh12和Sm14都能保护小鼠免受肝炎链球菌和曼氏链球菌的挑战性感染，所以它们可能具有可能涉及脂肪酸的常见保护性表位。进一步的研究正在进行中，以确定这些潜在的常见表位的化学性质。

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    《Journal of Parasitology》 |2001年第2期|p.00000426-00000428|共3页
  • 作者

    A. M. Espinoa and G. V. Hillyerab;

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    aLaboratory of Parasite Immunology and Pathology, University of Puerto Rico School of Medicine, San Juan, Puerto Rico 00936. bCorresponding author: Laboratory of Parasite Immunology & Pathology, Suite 617-A, University of Puerto Rico School of Medicine, P.O. Box 365067, San Juan, Puerto Rico 00936-5067;

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