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首页> 外文期刊>Journal of Plant Growth Regulation >Involvement of cis-Zeatin, Dihydrozeatin, and Aromatic Cytokinins in Germination and Seedling Establishment of Maize, Oats, and Lucerne
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Involvement of cis-Zeatin, Dihydrozeatin, and Aromatic Cytokinins in Germination and Seedling Establishment of Maize, Oats, and Lucerne

机译：顺式玉米素，二氢玉米素和芳香细胞分裂素参与玉米，燕麦和卢塞恩种子的萌发和幼苗生长

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摘要

The aims of this study were to monitor endogenous cytokinin levels during germination and early seedling establishment in oats, maize, and lucerne to determine which cytokinin forms are involved in these processes; to quantify the transfer ribonucleic acid (tRNA)-bound cytokinins; and to measure cytokinin oxidase/dehydrogenase (CKX) activity. Cytokinins were identified using UPLC-MS/MS. The predominant free cytokinins present in the dry seeds were dihydrozeatin-type (DHZ) in lucerne and maize and cZ-type (cis-zeatin) in oats. Upon imbibition, there was a large increase in cZ-type cytokinins in lucerne although the cZ-type cytokinins remained at high levels in oats. In maize, the high concentrations of DHZ-type cytokinins decreased prior to radicle emergence. Four tRNA-bound cytokinins [cis-zeatin riboside (cZR)N 6-(2-isopentenyl)adenosine (iPR), dihydrozeatin riboside (DHZR), trans-zeatin riboside (tZR)] were detected in low concentrations in all three species investigated. CKX activity was measured using an in vitro radioisotope assay. The order of substrate preference was N 6-(2-isopentenyl)adenine (iP)trans-zeatin (tZ)cZ in all three species, with activity fluctuating as germination proceeded. There was a negative correlation between CKX activity and iP concentrations and a positive correlation between CKX activity and O-glucoside levels. As O-glucosides are less resistant to CKX degradation, they may provide a readily available source of cytokinins that can be converted to physiologically active cytokinins required during germination. Aromatic cytokinins made a very small contribution to the total cytokinin pool and increased only slightly during seedling establishment, suggesting that they do not play a major role in germination.
机译：本研究的目的是监测燕麦，玉米和卢塞恩在发芽和幼苗早期建立过程中的内源性细胞分裂素水平，以确定哪些细胞分裂素形式参与这些过程。定量转移核糖核酸（tRNA）结合的细胞分裂素；并测量细胞分裂素氧化酶/脱氢酶（CKX）的活性。使用UPLC-MS / MS鉴定细胞分裂素。干燥种子中存在的主要游离细胞分裂素是卢塞恩和玉米中的二氢玉米蛋白型（DHZ）和燕麦中的cZ型（顺式玉米蛋白）。吸收后，尽管燕麦中cZ型细胞分裂素保持高水平，但卢塞恩中cZ型细胞分裂素有大量增加。在玉米中，高浓度的DHZ型细胞分裂素在胚根出现之前下降。在低浓度下检测到4种tRNA结合的细胞分裂素[顺式玉米素核糖苷（cZR）> N 6 -（2-异戊烯基）腺苷（iPR），二氢玉米素核糖苷（DHZR），反式玉米素核糖苷（tZR）]在所有三个物种中进行调查。使用体外放射性同位素测定法测量CKX活性。在所有三个物种中，底物优先顺序为N 6 -（2-异戊烯基）腺嘌呤（iP）>反式玉米素（tZ）> cZ，其活性随发芽的进行而波动。 CKX活性与iP浓度之间呈负相关，而CKX活性与O-葡萄糖苷水平呈正相关。由于O-葡萄糖苷对CKX降解的抵抗力较弱，因此它们可以提供易于获得的细胞分裂素来源，可以将其转化为发芽过程中所需的生理活性细胞分裂素。芳香族细胞分裂素对总细胞分裂素的贡献很小，在苗期建立过程中仅略有增加，表明它们在发芽中不发挥主要作用。

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  • 来源
    《Journal of Plant Growth Regulation》 |2012年第3期|p.392-405|共14页
  • 作者

    Wendy A. Stirk; Kateřina Václavíková; Ondřej Novák; Silvia Gajdošová; Ondřej Kotland; Václav Motyka; Miroslav Strnad; Johannes van Staden;

  • 作者单位

    Research Centre for Plant Growth and Development, School of Biological and Conservation Sciences, University of KwaZulu-Natal Pietermaritzburg, P/Bag X01, Scottsville, 3209, South Africa;

    Laboratory of Growth Regulators, Palacký University &amp Institute of Experimental Botany AS CR, Šlechtitelů 11, 78371, Olomouc, Czech Republic;

    Laboratory of Growth Regulators, Palacký University &amp Institute of Experimental Botany AS CR, Šlechtitelů 11, 78371, Olomouc, Czech Republic;

    Laboratory of Hormonal Regulations in Plants, Institute of Experimental Botany AS CR, Rozvojová 263, 16502, Prague 6, Czech Republic;

    Department of Biochemistry, Faculty of Science, Palacký University, Šlechtitelů 11, 78371, Olomouc, Czech Republic;

    Laboratory of Hormonal Regulations in Plants, Institute of Experimental Botany AS CR, Rozvojová 263, 16502, Prague 6, Czech Republic;

    Laboratory of;

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  • 正文语种 eng
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  • 关键词

    cis-Zeatin; Cytokinin oxidase/dehydrogenase; Dihydrozeatin; Lucerne; Maize; Oats; tRNA degradation;

    机译：顺式玉米素;细胞分裂素氧化酶/脱氢酶;二氢玉米素;卢塞恩;玉米;燕麦;tRNA降解;

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