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首页> 外文期刊>Journal of Polymers and the Environment >Fungal Degradation of the Bioplastic PHB (Poly-3-hydroxy-butyric acid)
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Fungal Degradation of the Bioplastic PHB (Poly-3-hydroxy-butyric acid)

机译：生物塑料PHB（聚-3-羟基丁酸）的真菌降解

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摘要

PHB (poly-3-hydroxybutyric acid) is a thermoplastic polyester synthesized by Ralstonia eu-tropha and other bacteria as a form of intracellular carbon and energy storage and accumulated as inclusions in the cytoplasm of these bacteria. The degradation of PHB by fungi from samples collected from various environments was studied. PHB depolymerization was tested in vials containing a PHB-containing medium which were inoculated with isolates from the samples. The degradation activity was detected by the formation of a clear zone below and around the fungal colony. In total, 105 fungi were isolated from 15 natural habitats and 8 lichens, among which 41 strains showed PHB degradation. Most of these were deuteromycetes (fungi imperfecti) resembling species of Penicillium and Aspergillus and were isolated mostly from soils, compost, hay, and lichens. Soil-containing environments were the habitats from which the largest number of fungal PHB degraders were found. Other organisms involved in PHB degradation were observed. A total number of 31 bacterial strains out of 67 isolates showed clear zones on assay medium. Protozoa, possible PHB degraders, were also found in several samples such as pond, soil, hay, horse dung, and lichen. Lichen, a fungi and algae symbiosis, was an unexpected sample from which fungal and bacterial PHB degraders were isolated.
机译：PHB（聚-3-羟基丁酸）是一种热塑性聚酯，由富营养小球藻和其他细菌以细胞内碳和能量存储的形式合成，并作为内含物累积在这些细菌的细胞质中。研究了从各种环境中收集的样品中真菌对PHB的降解作用。在装有含PHB的培养基的小瓶中测试PHB的解聚反应，并用样品中的分离物进行接种。通过在真菌菌落下方和周围形成一个透明区域来检测降解活性。总共从15个自然栖息地和8个地衣中分离出105种真菌，其中41株表现出PHB降解。其中大多数是类似于青霉属和曲霉属的氘菌（真菌不全），并且大多从土壤，堆肥，干草和地衣中分离出来。含有土壤的环境是发现最多数量的真菌PHB降解物的栖息地。观察到其他与PHB降解有关的生物。 67个分离物中的31个细菌菌株总数在分析培养基上显示出清晰的区域。原生动物，可能的PHB降解物，也在一些样品中发现，例如池塘，土壤，干草，马粪和地衣。地衣是一种真菌和藻类的共生体，是一种出乎意料的样品，可从中分离出真菌和细菌的PHB降解物。

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