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首页> 外文期刊>Journal of Polymers and the Environment >Effects of Poly(Ethylene Glycol) on the Production of Poly(β-Hydroxybutyrate) by Azotobacter vinelandii UWD
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Effects of Poly(Ethylene Glycol) on the Production of Poly(β-Hydroxybutyrate) by Azotobacter vinelandii UWD

机译：聚乙二醇对葡萄固氮菌UWD生产聚β-羟基丁酸酯的影响

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摘要

Azotobacter vinelandii UWD, ATCC 53799, an engineered strain derived from Azotobacter vinelandii UW was used in the poly(ethylene glycol) (PEG)-modulated synthesis of poly(p-hy-droxybutyrate) (PHB). To the best of our knowledge, this is the first report on modulating the production of PHB by amending the fermentation broth with PEG using A. vinelandii UWD. It was determined that A. vinelandii UWD is prone to back-mutation to the parent strain; hence fermentation experiments require the use of the antibiotic rifampicin. Diethylene glycol (DEG) and PEGs with molecular weights of 400, 2000, and 3400 Da and pentaerythritol ethoxylate (PEE) were used in the modulated fermentation experiments in a concentration of 2% (w/v). The molecular weight of the resulting polymers was reduced by up to 78%. No impact on the productivity of the strain was observed. Spectroscopic evidence showed that PEG-modulated synthesis resulted in the cova-lent attachment of the ethylene glycol moiety only when a small molecule, DEG, was used. PEGs had the same effects on the polymer formation in terms of molecular weight reduction as DEG, but no spectroscopic evidence was found for the formation of a covalent linkage between PHB and higher molecular weight PEGs.
机译：葡萄固氮菌UWD，ATCC 53799，一种来自葡萄固氮菌UW的工程菌株，用于聚（对羟基苯甲酸丁酯）（PHB）的聚乙二醇（PEG）调节的合成。据我们所知，这是关于通过使用A. vinelandii UWD用PEG修改发酵液来调节PHB产量的第一份报告。经确定，A。vinelandii UWD易于向亲本菌株回复突变。因此，发酵实验需要使用抗生素利福平。分子量为400、2000和3400 Da的二甘醇（DEG）和PEG和季戊四醇乙氧基化物（PEE）用于调制发酵实验，浓度为2％（w / v）。所得聚合物的分子量降低多达78％。没有观察到对菌株生产力的影响。光谱证据表明，只有当使用小分子DEG时，PEG调节的合成作用才导致乙二醇部分的空位连接。就分子量降低而言，PEG对聚合物的形成具有与DEG相同的作用，但没有发现光谱证据表明PHB与较高分子量PEG之间形成共价键。

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