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首页> 外文期刊>Journal of Polymers and the Environment >The Use of Azohydromonas lata DSM 1122 to Produce 4-hydroxyvalerate-Containing PolyhydroxyalkanoateTerpolymers, and Unique Polymer Blends from Mixed-Cultures with Burkholderia sacchari DSM 17165
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The Use of Azohydromonas lata DSM 1122 to Produce 4-hydroxyvalerate-Containing PolyhydroxyalkanoateTerpolymers, and Unique Polymer Blends from Mixed-Cultures with Burkholderia sacchari DSM 17165

机译：乳突孢子菌DSM 1122在生产4-羟基戊酸的聚羟基链烷酸酯三元共聚物中的应用，以及与糖味伯克霍尔德氏菌DSM 17165混合培养的独特聚合物共混物

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Azohydromonas lata DSM 1122 was utilized to synthesize short-chain-length (scl-) polyhydroxyalkanoate (PHA) terpolymers containing 3-hydroxybutyric (3HB) acid, 3-hydroxyvaleric (3HV) acid, and 4-hydroxyvaleric (4HV) acid from mixtures of glucose (GLC; 1 wt%) and levulinic acid (LevA; 0-0.4 wt%). LevA media concentrations greater than 0.4% completely inhibited cellular growth. At LevA concentrations aecurrency 0.4%, the 3HV polymer content remained constant (3-5 mol%). The 4HV content was two-fold higher in the polymers derived from the 0.2% LevA-containing cultures reaching a maximum of 9 mol% (vs. 4 mol% in the 0.4% LevA-containing cultures). Polymer molecular weights (based on number-average molecular weight, M-n) were smallest (M-n = 240,000 g/mol) when synthesized in the presence of 0.2% LevA. At 0.4% LevA and at 1% GLC the average M-n values were 43% and 87% larger than the polymers synthesized in the presence of 0.2% LevA, respectively. Mixed-cultures containing A. lata and Burkholderia sacchari DSM 17165, a known poly-3-hydroxybutyrate-block-3-hydroxyvalerate (P3HB-block-3HV) producer, using LevA media concentrations aecurrency 0.4% and staggered inoculations resulted in scl-PHA polymer mixtures with improved tensile properties. The results of this study show that LevA can be utilized in combination with simple sugars to produce unique scl-PHA terpolyesters and scl-PHA mixtures with enhanced properties.
机译：偶氮氢解单胞菌DSM 1122用于合成短链长（scl-）聚羟基链烷酸酯（PHA）三元共聚物，其中包含3-羟基丁酸（3HB）酸，3-羟基戊酸（3HV）酸和4-羟基戊酸（4HV）的混合物葡萄糖（GLC； 1重量％）和乙酰丙酸（LevA； 0-0.4重量％）。大于0.4％的LevA培养基浓度完全抑制了细胞生长。在LevA浓度ae货币为0.4％时，3HV聚合物的含量保持恒定（3-5mol％）。在衍生自含0.2％LevA的培养物的聚合物中，4HV含量高出两倍，达到最大9 mol％（相对于含0.4％LevA的培养物为4 mol％）。当在0.2％LevA存在下合成时，聚合物分子量（基于数均分子量，M-n）最小（M-n = 240,000 g / mol）。在0.4％LevA和1％GLC时，平均M-n值分别比在0.2％LevA存在下合成的聚合物大43％和87％。含有A. lata和Burkholderia sacchari DSM 17165（已知的聚3-羟基丁酸酯-嵌段-3-羟基戊酸酯（P3HB-block-3HV）生产商）的混合培养物，使用LevA培养基浓度ae 0.4％，并且交错接种导致具有改善的拉伸性能的scl-PHA聚合物混合物。这项研究的结果表明，LevA可以与简单的糖结合使用，以产生独特的scl-PHA三元聚酯和具有增强特性的scl-PHA混合物。

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