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首页> 外文期刊>Journal of Polymers and the Environment >Preparation of Chitooligosaccharide by Hydrogen Peroxide Degradation of Chitosan and Its Effect on Soybean Seed Germination
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Preparation of Chitooligosaccharide by Hydrogen Peroxide Degradation of Chitosan and Its Effect on Soybean Seed Germination

机译：壳聚糖过氧化氢降解氯寡糖的制备及其对大豆种子萌发的影响

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摘要

Chitooligosaccharides (COS) were prepared by hydrogen peroxide (1%) degradation of chitosan solution (3%) in dilute lactic acid at ambient temperature. The molecular weights of degraded chitosan were measured by gel permeation chromatography, and the chemical structure of resultant COS were characterized by Fourier transform infrared spectra. The decrease of chitosan Mw followed the first-order kinetics with the rate constant of 5.71x10(-7) mol/g/h. As a result, the COS with Mw of 4500g/mol was obtained at 360h of the reaction time and its chemical structure almost remained the same as initial chitosan. Germination ability of soybean seeds pre-soaking in COS (4500g/mol) solution with different concentrations, particularly 0 (control), 50, 100 and 200mg/L was investigated. Results indicated that germination rate increased significantly for all soybean seeds treated with COS compared to the control. Thus, COS prepared by this method can be potentially applied to large-scale production with reasonably low cost and used as an effective biostimulant for sustainable agriculture.
机译：通过在环境温度下在稀乳酸中的含氯烷溶液（3％）的过氧化氢（1％）降解的氯寡糖（1％）在环境温度下进行制备。通过凝胶渗透色谱法测量退化的壳聚糖的分子量，并通过傅里叶变换红外光谱表征了所得Co的化学结构。壳聚糖MW的减少跟随一阶动力学，速率常数为5.71x10（-7）mol / g / h。结果，在反应时间的360h中获得具有4500g / mol的Mw的COS，其化学结构几乎保持与初始壳聚糖相同。研究了用不同浓度，特别是0（对照），50,100和200mg / L的COS（4500g / mol）溶液预浸泡的大豆种子的发芽能力。结果表明，与对照相比，用COS处理的所有大豆种子发芽率显着增加。因此，通过该方法制备的COS可以潜在地应用于大规模生产，其成本相当低，用作可持续农业的有效生物诱色剂。

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