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首页> 外文期刊>Journal of Polymers and the Environment >Optimization of L(+) Lactic Acid Production from Solid Pineapple Waste (SPW) by Rhizopus oryzae NRRL 395
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Optimization of L(+) Lactic Acid Production from Solid Pineapple Waste (SPW) by Rhizopus oryzae NRRL 395

机译：Rhizopus Oryzae NRRL 395从固体菠萝废物（SPW）的L（+）乳酸产生的优化

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摘要

Solid pineapple waste (SPW) is one of the most abundant agricultural wastes found in the tropic region. Improper management could lead to deposition of such waste in the environment causing other problems. Optimized bioconversion into value added products using potent microorganism could be an option to manage SPW. In this study, Rhizopus oryzaeNRRL 395 was used to convert SPW to L(+) lactic acid through solid-state fermentation (SSF). The lactic acid production, reducing sugar utilization and by-product formation were studied using central composite design (CCD) after screening step using 2 level Factorial Design (2LFD). Studied parameters include moisture content (45 to 85% w/w), incubation time (0 to 7 days), temperature (25 to 45 degrees C), pH (4 to 8), and inoculum size (1 x 10(5) to 1 x 10(9) spores/g). During 2LFD, the highest lactic acid concentration obtained was 84.85 mg/g under the condition of 60% w/w of moisture content, 3 days of incubation, 35 degrees C, pH of 7 and 1 x 10(7) spores/g. Based on the condition suggested by CCD, the maximum lactic acid concentration of 103.69 mg/g was obtained with the following optimized SSF conditions: 67.53% w/w of moisture content, 3 days of incubation, temperature of 32.2 degrees C, pH of 5.6, and inoculum size of 1 x 10(7) spores/g.[GRAPHICS].
机译：坚固的菠萝废物（SPW）是热带地区最丰富的农业废物之一。管理不当可能导致在环境中沉积这种废物，导致其他问题。使用有效微生物的优化生物转化为增值产品可以是管理SPW的选项。在该研究中，Rhizopus OryzaenRRL 395用于通过固态发酵（SSF）将SPW转换为L（+）乳酸。使用2级阶乘设计（2LFD）在筛选步骤后使用中央复合设计（CCD）研究乳酸产生，降低糖利用和副产物。研究参数包括水分含量（45％至85％w / w），孵育时间（0至7天），温度（25至45℃），pH（4至8）和接种物尺寸（1 x 10（5）到1 x 10（9）孢子/ g）。在2LFD期间，在60％w / w的水分含量的条件下，得到的最高乳酸浓度为84.85mg / g，孵育3天，35℃，pH为7和1×10（7）孢子/ g。基于CCD建议的情况，通过以下优化的SSF条件获得103.69mg / g的最大乳酸浓度：67.53％w / w水分含量，孵育3天，温度为32.2℃，pH为5.6 ，接种尺寸为1×10（7）孢子/ g. [图形]。

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