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首页> 外文期刊>Journal of Polymers and the Environment >The Production of Polyhydroxybutyrate by Two-Step Fermentation and the Application of Polyhydroxybutyrate as a Novel Substrate fora Biolubricant
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The Production of Polyhydroxybutyrate by Two-Step Fermentation and the Application of Polyhydroxybutyrate as a Novel Substrate fora Biolubricant

机译：两步发酵生产聚羟基丁酸酯及其在新型生物润滑剂基质中的应用

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Two-step fermentation was utilized to increase PHB production. Cupriavidus necator was firstly cultivated in molasses medium for 144 h. Afterward, cells were recovered and extracted for PHB. The highest biomass (2.10 g/L) and PHB (62.38% of DCW) were obtained in the first reactor after 108 h of incubation. Filtrate after cell recovery were overflowed to the second reactor. Filtrate after cell recovery contained sugar and organic acids. Therefore, it was suitable to be re-utilized as substrate for PHB. In the second reactor, 10% of C. necator was inoculated and incubated for 120 h. The highest biomass (1.67 g/L) and PHB (67.66% of DCW) were achieved after 96 h of incubation. PHB was further utilized as substrate for biolubricant. The production of biolubricant involved a two-stage transesterification. The biolubricant from PHB passed the ISO VG-15 commercial standards for hydraulic systems and many other industrial applications.
机译：利用两步发酵来增加PHB的产量。首先在糖蜜培养基中将Cupriavidus necator培养144小时。之后，回收细胞并提取PHB。孵育108小时后，在第一个反应器中获得了最高的生物量（2.10 g / L）和PHB（DCW的62.38％）。细胞回收后的滤液溢流至第二反应器。细胞回收后的滤液中含有糖和有机酸。因此，适合重新用作PHB的底物。在第二个反应器中，接种10％的C.necator并孵育120小时。温育96小时后获得最高的生物量（1.67 g / L）和PHB（DCW的67.66％）。 PHB被进一步用作生物润滑剂的底物。生物润滑剂的生产涉及两个阶段的酯交换反应。 PHB的生物润滑剂通过了液压系统和许多其他工业应用的ISO VG-15商业标准。

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