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首页> 外文期刊>Journal of Polymers and the Environment >Kinetic Analysis of the Temperature Effect on Polyhydroxyalkanoate Production by Haloferax mediterranei in Synthetic Molasses Wastewater
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Kinetic Analysis of the Temperature Effect on Polyhydroxyalkanoate Production by Haloferax mediterranei in Synthetic Molasses Wastewater

机译：温度对合成糖蜜废水中介氧磷（Haloferax mediterranei）生产聚羟基链烷酸酯影响的动力学分析

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摘要

Haloferax mediterranei is an extremely halophilic archaeon that is able to synthesize polyhydroxyalkanoate (PHA) in high salt environment with low sterility demand. In this study, a mathematical model was validated and calibrated for describing the kinetic behavior of H. mediterranei at 15, 20, 25, and 35 A degrees C in synthetic molasses wastewater. Results showed that the production of PHA by H. mediterranei, ranging from 390 to 620 mg h(-1) L-1, was strongly dependent on the temperature. The specific growth rate (A mu (max)), specific substrate utilization rate (q (max)), and specific decay rate (k (d)) of H. mediterranei increased with temperature following Arrhenius equation prediction. The estimated activation energy was 58.31, 25.59, and 22.38 kJ mol(-1) for the process of cell growth, substrate utilization, and cell decay of H. mediterranei, respectively. The high temperature triggered the increased PHA storage even without nitrogen limitation. Thus, working at high temperatures seems a good strategy for improving the PHA productivity of H. mediterranei.
机译：地中海嗜盐菌（Haloferax mediterranei）是一种极嗜盐的古细菌，它能够在高盐环境中以低无菌需求合成聚羟基链烷酸酯（PHA）。在这项研究中，数学模型经过验证和校准，以描述合成糖蜜废水中嗜水链球菌在15、20、25和35 A摄氏度下的动力学行为。结果表明，嗜中性链球菌产生的PHA范围从390到620 mg h（-1）L-1，强烈依赖于温度。根据Arrhenius方程预测，地中海中的嗜水杆菌的比生长速率（A mu（max）），比底物利用率（q（max））和比衰减速率（k（d））随着温度的升高而增加。估计的活化能分别为58.31、25.59和22.38 kJ mol（-1），用于细胞生长，底物利用率和嗜中性链球菌的细胞衰变过程。即使没有氮限制，高温也导致PHA存储增加。因此，在高温下工作似乎是提高地中海嗜血杆菌的PHA生产率的好策略。

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