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Municipal Wastes Treatment and Production of Polyhydroxyalkanoate by Modified Two-Stage Batch Reactor

机译:改进的两级间歇式反应器处理城市垃圾及生产聚羟基链烷酸酯

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摘要

The municipal wastes were utilized as substrate for polyhydroxyalkanoate (PHA) using two strains of Bacillus licheniformis (PHAs-007, wild type and M2-12, mutant). Municipal wastes were subjected to separate wastewater and biosolid. Municipal biosolid was digested by anaerobic bacteria thereafter only the supernatant with soluble organic compounds was subjected into the PHA-producing reactor containing municipal wastewater. The mutant strain M2-12 gave the highest value of biomass (42.0 ± 2.0 g/L) and PHA concentration (37.4 ± 1.0 g/L with 88.9 % of dry cell weight, DCW) and reduced 76.5 % of soluble chemical oxygen demand after 60 h of cultivation. The value of pH, biochemical oxygen demand and total solid of the reclaimed wastewater after PHA recovery was 7.1, 20 and 97 mg/L, respectively. Moreover, the polymers produced by both strains of B. licheniformis were characterized. The resultant polymer from B. licheniformis PHAs-007 and M2-12 cultivated in the PHA-producing reactor was identified as poly-3-hydroxybutyrate-co-3-hy-droxyvalerate [P(3HB-co-3HV)] and poly-3-hydroxybuty-rate-co-4-hydroxybutyrate [P(3HB-co-4HB)], respectively. The results suggesting that the production of PHA by municipal wastes is feasible thus the PHA production stage can be integrated in waste treatment to produce PHA and treated municipal wastes at the same time.
机译:使用两种地衣芽孢杆菌菌株(PHAs-007,野生型和M2-12,突变体),将市政废物用作聚羟基链烷酸酯(PHA)的底物。城市垃圾分别受到废水和生物固体的处理。用厌氧菌消化市政生物固体,然后仅将含有可溶性有机化合物的上清液放入含有市政废水的PHA生产反应器中。突变菌株M2-12具有最高的生物量(42.0±2.0 g / L)和PHA浓度(37.4±1.0 g / L,干细胞重量为88.9%,DCW)最高,可溶性化学需氧量降低了76.5%。栽培60小时。 PHA回收后的再生废水的pH值,生化需氧量和总固体分别为7.1、20和97 mg / L。此外,表征了由两种地衣芽孢杆菌产生的聚合物。在产PHA的反应器中培养的地衣芽孢杆菌PHAs-007和M2-12产生的聚合物被鉴定为聚3-羟基丁酸酯-co-3-羟基丙酸酯[P(3HB-co-3HV)]和聚3-羟基丁酸酯-co-4-羟基丁酸酯[P(3HB-co-4HB)]。结果表明,利用城市垃圾生产PHA是可行的,因此PHA生产阶段可以整合到废物处理中,以生产PHA和同时处理的城市垃圾。

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