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首页> 外文期刊>Journal of porous materials >A comparative study on mesocellular foam silica with different template removal methods and their effects on enzyme immobilization
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A comparative study on mesocellular foam silica with different template removal methods and their effects on enzyme immobilization

机译：具有不同模板去除方法的Mesocellular Foam二氧化硅的比较研究及其对酶固定的影响

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Mesocellular foam silica (MCF) was synthesized using Pluronic P-123 triblock copolymer as a template. Calcination and solvent extraction were employed to remove templates and compared. Through physical, chemical and functional characterization interesting results have been observed. Microstructures of the two MCFs were very different. Pore structure of the calcined MCF was found to be more ordered than that of the solvent extracted MCF. However, pores of the solvent extracted MCF seemed more defined and spongy under SEM observations. Surface functional groups and their concentrations characterized by ToF-SIMS were also found to be different. Surface silanol groups were found to be more concentrated in the calcined MCF. Each type of MCFs was subsequently grafted by an amine functional group, adsorbed by Au ion precursors, and reduced to form Au nanoparticles (AuNPs) on the MCF surfaces. These decorated MCF surfaces were immobilized with enzyme acetylcholinesterase (AChE), modified on screen-printed carbon electrodes and tested for electrochemical responses with acetylthiocholine. AuNPs were successfully distributed within the pores for both types of MCFs. Before the functional biosensor electrochemical tests, calcination seemed more promising than solvent extraction due to periodic structure of pores, high surface area, less contamination and higher concentration of surface silanol groups available for grafting of the amine functional group. However, after immobilization of the AuNPs for electron transport improvement and enzyme immobilization, a few observations of biosensing performances were noteworthy. Firstly, having AuNPs distributed throughout the MCF structure did not help the electron transport. Results of reduced response currents from both types of MCFs compared to MCFs without AuNPs indicated that MCF walls were too thick to allow electron transport. Secondly, the higher concentration of surface silanol groups in calcined MCFs resulted in Ostwald's ripening of small AuNPs giving bigger AuNPs than the case of solvent extraction. Therefore, although the AuNPs in enzyme biosensors are expected to help electron transport and enzyme immobilization, this study showed that although the former did not occur, the latter was well demonstrated. The smaller AuNPs in the solvent extraction case resulted in higher surface area than the bigger AuNPs in the calcination case. This was the reason why the response current was higher for the solvent extracted MCFs compared to the calcined MCFs.
机译：使用Pluronic P-123三嵌段共聚物作为模板合成Mesocellular泡沫二氧化硅（MCF）。使用煅烧和溶剂萃取来除去模板并比较。通过物理，化学和功能表征有趣的结果。两个MCF的微观结构非常不同。发现煅烧MCF的孔结构比溶剂提取的MCF更新。然而，溶剂的孔提取的MCF似乎在SEM观察下更具定义和海绵状。还发现表面官能团及其表征其特征的浓度不同。发现表面硅烷醇基团在煅烧的MCF中更浓缩。随后被胺官能团接枝的每种类型的MCF，被Au离子前体吸附，并还原以在MCF表面上形成Au纳米颗粒（AUNP）。将这些装饰的MCF表面固定用酶乙酰胆碱酯酶（ACHE）固定，在丝网印刷的碳电极上修饰并用乙酰硫代胆碱进行电化学响应。 AUNPS成功分布在毛孔内，用于两种类型的MCF。在功能性生物传感器电化学测试之前，由于孔隙，高表面积，较少污染和可用于接枝胺官能团的接枝孔的周期性结构，煅烧似乎比溶剂萃取更高。然而，在固定AUNPS进行电子传输改善和酶固定后，对生物沉积性表现的一些观察值得注意。首先，在整个MCF结构中分布的AUNP没有帮助电子传输。与没有AUNPS的MCF相比，两种类型MCF的响应电流降低结果表明MCF墙体过于厚，以允许电子传输。其次，煅烧MCF中较高浓度的表面硅烷醇基团导致OSTWALD的小肛周成熟，比溶剂萃取的情况更大。因此，虽然酶生物传感器中的肛门血管有助于电子运输和酶固定化，但这项研究表明，虽然前者没有发生，但后者很好地证明。溶剂提取壳体中的较小的AUNP导致煅烧壳体中的较大的AUNPS更高的表面积。这就是为溶剂提取的MCF与煅烧的MCF相比，响应电流更高的原因。

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