首页> 外文期刊>Journal of Scientific & Industrial Research >Production Characteristics, Activity Patterns and Biodecolourisation Applications of Thermostable Laccases from Corynebacterium Efficiens and Enterobacter Ludwigii
【24h】

Production Characteristics, Activity Patterns and Biodecolourisation Applications of Thermostable Laccases from Corynebacterium Efficiens and Enterobacter Ludwigii

机译:高效棒杆菌和路德维氏肠杆菌的热稳定漆酶的生产特性,活性模式和生物脱色应用

获取原文
获取原文并翻译 | 示例
           

摘要

The production conditions, characteristics and dye decolourisation potentials of two purified bacterial laccases from Corynebacterium efficiens and Enterobacter ludwigii were investigated. Optimum production pH was 3.0 and 4.0 respectively for E. ludwigii and C. efficiens. Khaya sp. and Gmelina arborea wood shavings, (NH4)(2)SO4 (30.2 mu M/ml) and K+ maximally supported laccase production in both organisms. The Vmax and specificity constants for both purified laccases were 44.6 mu M/mg; 1858.4S(-1)mu M-1 and 23.15 mu M/mg; 1015.4S(-1)mu M-1 respectively. Maximum laccase activity was between 80 degrees C and 90 degrees C and pH 7.0. In both laccases, Fe3+ induced higher activity and red Dylon dye was 69% and 64% decolourised. The properties exhibited by these laccases could enhance their suitability in diverse biotechnological applications.
机译:研究了两种高效棒杆菌和路德维氏肠杆菌的纯化细菌漆酶的生产条件,特征和染料脱色的潜力。 Ludwigii和C. efficiens的最佳生产pH分别为3.0和4.0。 Khaya sp。和Gmelina arborea刨花,(NH4)(2)SO4(30.2μM / ml)和K +在两种生物中最大程度地支持了漆酶的生产。两种纯化的漆酶的Vmax和特异性常数均为44.6μM/ mg。 1858.4S(-1)μM-1和23.15μM / mg; 1015.4S(-1)μM-1。最大漆酶活性在80℃至90℃和pH 7.0之间。在两种漆酶中,Fe3 +均具有较高的活性,红色Dylon染料的脱色率为69%,而64%的脱色为64%。这些漆酶表现出的特性可以增强其在各种生物技术应用中的适用性。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号