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首页> 外文期刊>Journal of soil & sediments >Effect of treated farm dairy effluents, with or without animal urine, on nitrous oxide emissions, ammonia oxidisers and denitrifiers in the soil
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Effect of treated farm dairy effluents, with or without animal urine, on nitrous oxide emissions, ammonia oxidisers and denitrifiers in the soil

机译:处理过的有或没有动物尿液的农场奶类废水对土壤中一氧化二氮排放,氨气氧化剂和反硝化剂的影响

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Purpose In New Zealand, the application of farm dairy effluent (FDE) on pasture soils is the third largest source of nitrous oxide (N2O) emissions from grazed grassland. Recently, new FDE treatment technologies have been developed to produce clarified water (CW) and treated effluent (TE) to recycle water and reduce the volume of fresh water used at the farm dairy. The aim of this study was to compare the effects of CW and TE with those of FDE on N2O emissions and the growth of ammonia-oxidising bacteria (AOB), ammonia-oxidising archaea (AOA) and denitrifiers, when the effluents were applied to a grazed pasture soil.Materials and methods A microcosm incubation study was carried out to determine the effects of applying CW, TE and untreated FDE, with or without animal urine, on N2O emissions, and the abundance of AOB, AOA and the denitrifying functional genes, including nirS, nirK and nosZ. The soil used was a Templeton silt loam (Udic Haplustepsts). The effluents were applied at nitrogen (N) rates equivalent to 100 kg N ha(-1) and the animal urine at 700 kg N ha(-1). The soils were incubated at 12 degrees C to simulate autumn/winter soil temperatures in New Zealand, and the soil moisture was maintained at field capacity.Results and discussion Results showed that the application of all the different effluents significantly increased the total N2O emissions (0.21-0.28 kg N2O-N ha(-1)) compared with that in the control (0.18 kg N2O-N ha(-1)). However, there were no significant differences in total N2O emissions between the different effluent treatments. Similarly, although the application of animal urine together with the different effluents further increased N2O emissions (7.7-8.8 kg N2O-N ha(-1)) above that from the urine only treatment (5.8 kg N2O-N ha(-1)), there were no significant differences among the different effluent plus urine treatments. These N2O results corresponded with the changing trends of the abundance of AOB, AOA, nirS, nirK and nosZ, that is the application of the CW, TE and FDE, with or without animal urine, had a similar impact on the growth dynamics of these microbial populations.Conclusions These results indicate that the application of the CW and TE to dairy pasture soils would have a similar effect on N2O emissions, ammonia oxidisers and denitrifiers as that of the untreated FDE, with or without animal urine. The treated effluent or clear water from the new effluent treatment technology would therefore not increase N2O emissions nor adversely affect the key microbial populations involved in N cycling in soil.
机译:目的在新西兰,在牧场土壤上施用农用奶类废水(FDE)是放牧草地的第三大一氧化二氮(N2O)排放源。最近,已经开发了新的FDE处理技术,以生产澄清水(CW)和处理后的废水(TE)以循环水并减少农场乳制品中使用的淡水量。这项研究的目的是比较将废水用于污水处理厂时,CW和TE与FDE对N2O排放以及氨氧化细菌(AOB),氨氧化古细菌(AOA)和反硝化剂的生长的影响。材料和方法进行了微观温育研究,以确定在有或没有动物尿液的情况下使用连续水,TE和未经处理的FDE对N2O排放以及AOB,AOA和反硝化功能基因的丰度的影响,包括nirS,nirK和nosZ。使用的土壤是邓普顿粉质壤土(Udic Haplustepsts)。以等于100千克N ha(-1)的氮(N)速率施用污水,以700千克N ha(-1)施用动物尿液。将土壤在12摄氏度下温育以模拟新西兰的秋冬土壤温度,并且将土壤水分保持在田间持水量。结果与讨论结果表明,所有不同废水的施用均显着增加了N2O的总排放量(0.21 -0.28 kg N2O-N ha(-1))与对照组的0.18 kg N2O-N ha(-1)比较。但是,不同废水处理之间的N2O总排放量没有显着差异。同样,尽管将动物尿液与不同的废水一起使用,仍比仅尿液处理(5.8 kg N2O-N ha(-1))进一步增加了N2O排放量(7.7-8.8 kg N2O-N ha(-1))。 ,不同的废水加尿液处理之间没有显着差异。这些N2O结果与AOB,AOA,nirS,nirK和nosZ的丰度变化趋势相对应,也就是说,无论有无动物尿液,连续波,TE和FDE的施用对这些动物的生长动力学都有相似的影响。结论这些结果表明,在有或没有动物尿液的情况下,将CW和TE应用于奶牛牧场土壤对N2O排放,氨氧化和反硝化剂的影响与未经处理的FDE相似。因此,采用新的污水处理技术处理过的污水或清水不会增加N2O排放,也不会不利地影响土壤中氮循环的关键微生物种群。

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