Optimized protocol for the biocompatibility testing of compression stockings and similar products with close skin contact in vitro

Wiegand Cornelia; Hansen Tanja; Koehnlein Johanna; Exner Ines; Damisch-Pohl Marlen; Schott Peter; Kruhner-Wiesenberger Ulrike; Hipler Uta-Christina; Pohlen Ernst

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Optimized protocol for the biocompatibility testing of compression stockings and similar products with close skin contact in vitro

机译：优化的方案，用于紧密接触皮肤的压力袜和类似产品的生物相容性测试

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Biocompatibility of six different compression stockings and cytotoxic effects were determined using HaCaT keratinocytes, L929 mouse fibroblasts, primary adult and juvenile keratinocytes Cells were quantified using a luminometric ATP assay and the photometric BCA test. Cytotoxic effects were determined by LDH release. An area-based extraction ratio of 1.25cm(2):mL could be shown to be superior to the weight-based extraction of test material. Extraction medium should be an acidic sweat solution as this helps to recreate in vivo conditions. Monolayer cultures of HaCaT keratinocytes or L929 mouse fibroblasts should be used for testing. Primary adult keratinocytes or primary juvenile keratinocytes can also be used. For the latter, testing under DMEM with FCS is recommended to achieve comparable results. It was found that the compression stockings tested exhibited no negative influence on cell viability in vitro and no direct cytotoxic effects measured as release of LDH. Hence, good biocompatibility could be asserted.

机译：使用HaCaT角质形成细胞，L929小鼠成纤维细胞，原代成年和幼年角质形成细胞确定六种不同压迫长袜的生物相容性和细胞毒性作用。使用光度ATP分析和光度BCA测试对细胞进行定量。细胞毒性作用通过LDH释放来确定。 1.25 cm（2）：mL的基于面积的提取比率可能会优于基于重量的测试材料的提取。提取介质应为酸性汗液，因为这有助于重建体内条件。测试应使用HaCaT角质形成细胞或L929小鼠成纤维细胞的单层培养。也可以使用原代成年角质形成细胞或幼年成年角质形成细胞。对于后者，建议在带有FCS的DMEM下进行测试以达到可比的结果。发现测试的压力袜在体外对细胞活力没有显示负面影响，并且没有测量到作为LDH释放的直接细胞毒性作用。因此，可以断言良好的生物相容性。

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来源
《The Journal of the Textile Institute》 |2018年第7期|891-902|共12页
作者
Wiegand Cornelia; Hansen Tanja; Koehnlein Johanna; Exner Ines; Damisch-Pohl Marlen; Schott Peter; Kruhner-Wiesenberger Ulrike; Hipler Uta-Christina; Pohlen Ernst;
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作者单位

Univ Hosp Jena, Dept Dermatol, Jena, Germany;

Fraunhofer ITEM, Hannover, Germany;

HygCen Germany GmbH, Schwerin, Germany;

Bauerfeind AG, Zeulenroda Triebes, Germany;

Bauerfeind AG, Zeulenroda Triebes, Germany;

Medi GmbH & Co KG, Bayreuth, Germany;

Sigvaris AG, St Gallen, Switzerland;

Univ Hosp Jena, Dept Dermatol, Jena, Germany;

Gutezeichengemeinschaft Med Kompressionsstrumpfe, Duren, Germany;

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正文语种 eng
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关键词
Biocompatibility; compression stocking; cytotoxicity; DIN EN ISO 10993-5; fibroblasts; keratinocytes;

机译：生物相容性;压力储存;细胞毒性;DIN EN ISO 10993-5;成纤维细胞;角质形成细胞;

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Optimized protocol for the biocompatibility testing of compression stockings and similar products with close skin contact in vitro

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