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首页> 外文期刊>The Journal of the Textile Institute >Enhancement reactivity of Bombyx mori silk fibroins via genipin-mediated grafting of a tyrosine-rich polypeptide
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Enhancement reactivity of Bombyx mori silk fibroins via genipin-mediated grafting of a tyrosine-rich polypeptide

机译：通过Genipin介导的富含酪氨​​酸多肽的嫁接增强家蚕丝纤蛋白的反应性

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摘要

Bombyx mori silk fibroin has potential applications in the biomedical field owing to its satisfactory biocompatibility. In the present work, a tyrosine-rich polypeptide (P) with the sequence of GKGYGGYGK was grafted onto fibroin molecules using a natural cross-linking agent of genipin (GP), aiming at improving the reactivity of the fibroin-based materials. Incubation of the polypeptide-grafted fibroin membrane was subsequently carried out, with a mushroom tyrosinase (MT) and a model functional macromolecule of epsilon-poly-l-lysine (epsilon-PL). The changes in the structure and composition of the silk fibroin before and after polypeptide grafting were examined by means of size exclusion chromatography and amino acid analysis. The results indicated that the polypeptide was covalently bonded to fibroin chains and evidently promoted the succeeding enzymatic coupling of epsilon-PL with fibroins, resulting in a higher content of epsilon-PL compared to the control. The obtained antibacterial activities against S. aureus and E. coli for the fibroin membrane treated with GP/P and MT/epsilon-PL was more satisfactory than others. Cytotoxicity testing reveals that polypeptide grafting did less impact on the biocompatibility of silk fibroins. The present work provides a novel method to improve the reactivity of silk fibroins and it can be utilized for bio-functionalization of silk fibroin.
机译：由于其令人满意的生物相容性，家蚕丝素蛋白在生物医学领域具有潜在的应用。在目前的工作中，使用天然的京尼平（GP）交联剂，将具有GKGYGGYGK序列的富含酪氨​​酸的多肽（P）嫁接到纤蛋白分子上，旨在改善基于纤蛋白的材料的反应性。随后用蘑菇酪氨酸酶（MT）和ε-聚-1-赖氨酸的模型功能大分子（ε-PL）对多肽移植的纤维蛋白膜进行孵育。通过尺寸排阻色谱法和氨基酸分析检查了丝素蛋白在多肽接枝之前和之后的结构和组成的变化。结果表明该多肽共价键合至丝心蛋白链，并且明显促进了ε-PL与丝心蛋白的成功酶促偶联，与对照相比，导致更高的ε-PL含量。 GP / P和MT / epsilon-PL处理的丝蛋白膜对金黄色葡萄球菌和大肠埃希菌的抗菌活性比其他方法更令人满意。细胞毒性测试表明，多肽接枝对丝素蛋白的生物相容性影响较小。本工作提供了一种提高丝素蛋白反应性的新方法，可用于丝素蛋白的生物功能化。

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  • 来源
    《The Journal of the Textile Institute》 |2017年第12期|2115-2122|共8页
  • 作者

    Zhu Xueke; Wang Ping; Cui Li; Yu Yuanyuan; Wang Qiang; Fan Xuerong;

  • 作者单位

    Jiangnan Univ, Key Lab Sci & Technol Eco Text, Minist Educ, Wuxi, Peoples R China;

    Jiangnan Univ, Key Lab Sci & Technol Eco Text, Minist Educ, Wuxi, Peoples R China;

    Jiangnan Univ, Key Lab Sci & Technol Eco Text, Minist Educ, Wuxi, Peoples R China;

    Jiangnan Univ, Key Lab Sci & Technol Eco Text, Minist Educ, Wuxi, Peoples R China;

    Jiangnan Univ, Key Lab Sci & Technol Eco Text, Minist Educ, Wuxi, Peoples R China;

    Jiangnan Univ, Key Lab Sci & Technol Eco Text, Minist Educ, Wuxi, Peoples R China;

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  • 原文格式 PDF
  • 正文语种 eng
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  • 关键词

    Silk fibroin; genipin; tyrosine-rich polypeptide; tyrosinase; enzymatic reactivity;

    机译：丝素蛋白;Genipin;富含酪氨酸的多肽;酪氨酸酶;酶反应性;

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