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首页> 外文期刊>The Journal of the Textile Institute >Electrospun zein/PVA fibrous mats as three-dimensional surface for embryonic stem cell culture
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Electrospun zein/PVA fibrous mats as three-dimensional surface for embryonic stem cell culture

机译：电纺玉米醇溶蛋白/ PVA纤维垫作为三维表面用于胚胎干细胞培养

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摘要

This paper reports a new method of producing electrospun zein/polyvinyl alcohol (PVA) mats as three-dimensional (3D) cell culture surface material. The electrospun structure has many advantages and protein-based biomaterials possess unique properties preferred for cell biocompatibility. However, electrospun fiber matrices developed from proteins have poor mechanical properties and morphological stability in the aqueous environments required for cell culture. Efforts have been made to improve the properties of electrospun protein scaffolds, including dry mechanical for handling and surface hydrophilicity, but the current methods have major limitations, such as cytotoxicity and low efficiency. In this research, experimental data showed that the adding different proportion of PVA influenced the properties of the zein mats. Considering the properties of the mats and mouse embryonic stem cells growth behavior on the various electrospun mats, the EM3 (zein/PVA with mass ratios of 3:1) showed better growth status than any other mats. EM3 affixed to cover-glass could be autoclaved for 30min at 120℃. In addition, embryonic stem cells cultured on chosen electrospun zein/PVA mats in vitro proved that culture products were easily attached onto mat and mat could be used for induction of stem cell differentiation and therefore promising mats for 3D cell culture surface applications.
机译：本文报道了一种新的生产电纺玉米蛋白/聚乙烯醇（PVA）垫材的新方法，该垫材是三维（3D）细胞培养表面材料。电纺结构具有许多优点，基于蛋白质的生物材料具有细胞生物相容性优选的独特特性。然而，由蛋白质形成的电纺纤维基质在细胞培养所需的水性环境中具有较差的机械性能和形态稳定性。已经努力改善电纺蛋白支架的性能，包括用于处理的干机械性能和表面亲水性，但是当前的方法具有主要局限性，例如细胞毒性和低效率。在这项研究中，实验数据表明添加不同比例的PVA会影响玉米蛋白垫的性能。考虑到垫子的特性以及在各种电纺垫子上小鼠胚胎干细胞的生长行为，EM3（玉米蛋白/ PVA的质量比为3：1）显示出比其他垫子更好的生长状态。贴在玻璃盖上的EM3可以在120℃下高压灭菌30分钟。此外，体外在选定的静电纺玉米蛋白/ PVA垫上培养的胚胎干细胞证明，培养产物易于附着在垫上，并且垫可用于诱导干细胞分化，因此有望用于3D细胞培养表面应用。

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  • 来源
    《The Journal of the Textile Institute》 |2014年第3期|246-255|共10页
  • 作者

    Mei Zhang; Yongjia Liu; Hualin Yi; Jiashuang Luan; Yan Zhang; Hongli Cai; Dahui Sun;

  • 作者单位

    College of Quartermaster Technology, Jilin University, Changchun, China;

    College of Quartermaster Technology, Jilin University, Changchun, China;

    Key Laboratory of Gene Engineering of the Ministry of Education, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, China;

    College of Quartermaster Technology, Jilin University, Changchun, China;

    Key Laboratory of Gene Engineering of the Ministry of Education, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, China;

    College of Quartermaster Technology, Jilin University, Changchun, China;

    Norman Bethune First Hospital, Jilin University, Changchun, China;

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  • 正文语种 eng
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  • 关键词

    zein; electrospinning; cell culture surface; fibrous mats; embryonic stem cell;

    机译：玉米蛋白电纺;细胞培养表面;纤维垫胚胎干细胞;

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