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首页> 外文期刊>The Journal of the Textile Institute >The potential use of alkaline protease from Streptomyces albidoflavus as an eco-friendly wool modifier
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The potential use of alkaline protease from Streptomyces albidoflavus as an eco-friendly wool modifier

机译：链霉菌碱性蛋白酶作为生态友好型羊毛改良剂的潜在用途

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摘要

The main task of this study is to search for the proper conditions of the enzymatic treatment using an alkaline bacterial protease, as an eco-friendly option, to improve some performance properties and dyeability of grey wool fabric with acid dyes. The efficiency of the enzymatic treatment has been improved by prewashing in the presence of a nonionic wetting agent alone and in combination with H_2O_2. The enhancement in wool performance proper ties as well as in the extent of post-acid dyeing is determined by pretreatment regime, enzyme dosage, as well as enzymatic treatment conditions. For modified wool fabric samples, as the weight loss increases, the nitrogen con tent decreases, the whiteness index as well as the dyeability with the acid dye improves compared with the pre washed fabric samples. Proper conditions for maximising the efficiency of enzymatic treatment along with attaining better performance properties and minimal wool damage are: prewashing in the presence of a nonionic surfactant along with H_2O_2 followed by enzymatic treatment using the enzyme dosage (3 ml/g fabric), pH (9), incubation time (45 min), temperature (40 ℃), LR (1/20) and rotational speed (40 rpm). The enzyme inactivation was performed at 100 ℃ for 5 min, followed by thoroughly rinsing, neutralising and air-drying before testing. SEM images also provide evidence that pretreatment of the wool substrate enhanced the proteolysis of cuticle lay ers, their degradation and potential removal by the subsequent protease treatment.
机译：这项研究的主要任务是寻找一种使用碱性细菌蛋白酶进行酶处理的合适条件，这是一种生态友好的选择，以改善灰羊毛织物用酸性染料的某些性能和可染性。通过在单独的非离子型润湿剂和与H_2O_2结合的非离子型润湿剂的存在下进行预洗涤，可以提高酶处理的效率。羊毛性能的适当性以及后酸染程度的提高取决于预处理方案，酶的用量以及酶处理条件。对于改性羊毛织物样品，与预洗涤的织物样品相比，随着重量损失的增加，氮含量降低，白度指数以及用酸性染料的可染性得到改善。使酶处理效率最大化并获得更好的性能和最小的羊毛损伤的合适条件是：在非离子表面活性剂和H_2O_2的存在下进行预洗涤，然后使用酶剂量（3 ml / g织物），pH（ 9），孵育时间（45分钟），温度（40℃），LR（1/20）和转速（40 rpm）。在100℃下进行5分钟的酶灭活，然后彻底漂洗，中和并风干，然后进行测试。 SEM图像还提供了证据，即羊毛基质的预处理增强了表皮层的蛋白水解，它们的降解和随后的蛋白酶处理的潜在去除作用。

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