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首页> 外文期刊>Journal of the American Chemical Society >Dissecting the Strand Folding Orientation and Formation of G-Quadruplexes in Single- and Double-Stranded Nucleic Acids by Ligand-lnduced Photocleavage Footprinting
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Dissecting the Strand Folding Orientation and Formation of G-Quadruplexes in Single- and Double-Stranded Nucleic Acids by Ligand-lnduced Photocleavage Footprinting

机译：配体诱导的光裂解足迹分析单链和双链核酸中链折叠方向和G四联体的形成。

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摘要

The widespread of G-quadruplex-forming sequences in genomic DNA and their role in regulating gene expression has made G-quadruplex structures attractive therapeutic targets against a variety of diseases, such as cancer. Information on the structure of G-quadruplexes is crucial for understanding their physiological roles and designing effective drugs against them. Resolving the structures of G-quadruplexes, however, remains a challenge especially for those in double-stranded DNA. In this work, we developed a photocleavage footprinting tech- nique to determine the folding orientation of each individual G-tract in intramolecular G-quadruplex formed in both single- and double-stranded nucleic acids. Based on the differential photocleavage induced by a ligand tetrakis(2-trimethylaminoethylethanol) phthalocyaninato zinc tetraiodine (Zn-TTAPc) to the guanines between the two terminal G-quartets in a G-quadruplex, this method identifies the guanines hosted in each terminal G-quartets to reveal G-tract orientation. The method is extremely intuitive, straightforward, and requires little expertise. Besides, it also detects G-quadruplex formation in long single- and double-stranded nucleic acids.
机译：G-四链体形成序列在基因组DNA中的广泛分布及其在调节基因表达中的作用已使G-四链体结构成为对抗多种疾病（例如癌症）的有吸引力的治疗靶标。有关G-四链体结构的信息对于理解它们的生理作用和设计针对它们的有效药物至关重要。然而，特别是对于双链DNA中的那些，解析G-四链体的结构仍然是一个挑战。在这项工作中，我们开发了一种光裂解足迹技术，以确定在单链和双链核酸中形成的分子内G-四链体中每个单独G链的折叠方向。基于配体四（2-三甲基氨基乙基乙醇）酞菁锌到四碘锌锌（Zn-TTAPc）对G-四链体中两个末端G四联体之间的鸟嘌呤诱导的差异光裂解，该方法确定了每个末端G-中的鸟嘌呤四重奏以揭示G谱线的方向。该方法非常直观，简单，几乎不需要专业知识。此外，它还能检测长单链和双链核酸中的G-四链体形成。

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  • 来源
    《Journal of the American Chemical Society》 |2011年第5期|p.1475-1483|共9页
  • 作者

    Ke-wei Zheng; Dan Zhang; Li-xia Zhang; Yu-hua Hao; Xiang Zhou; Zheng Tan;

  • 作者单位

    State Key Laboratory of Biomembrane and Membrane Biotechnology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, People's Republic of China;

    College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, People's Republic of China;

    College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, People's Republic of China;

    State Key Laboratory of Biomembrane and Membrane Biotechnology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, People's Republic of China;

    College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, People's Republic of China;

    State Key Laboratory of Biomembrane and Membrane Biotechnology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, People's Republic of China;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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