Evaluation of Solution Phase Isoelectrofocusing as Part of Proteomics Strategies

Yanming AN; Zongming FU; Catherine FENSELAU

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Evaluation of Solution Phase Isoelectrofocusing as Part of Proteomics Strategies

机译：蛋白质组学策略的溶液相等电聚焦评估

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Solution isoelectrofocusing has been evaluated for prefractionation of nuclear proteins from human cancer MCF-7 cells. Two separation devices were exercised using commercial polyacrylamide membranes, which carry embedded immobiline mixtures to establish pH boundaries between chambers. Protein recovery was quantified and 2-D gel electrophoresis was used to evaluate resolution. Solution isoelectrofocusing provides satisfactory separations and decreases the complexity of the protein mixture, however rates of recovery need to be improved.

机译：已对溶液等电聚焦进行了评估，以预分离来自人类癌症MCF-7细胞的核蛋白。使用市售的聚丙烯酰胺膜对两个分离装置进行了测试，该膜携带嵌入的固定混合物，以在各腔室之间建立pH边界。定量蛋白质回收率，并使用二维凝胶电泳评估分离度。溶液等电聚焦可提供令人满意的分离效果并降低蛋白质混合物的复杂性，但是需要提高回收率。

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《Journal of the Mass Spectrometry Society of Japan》 |2005年第1期|p.1-6|共6页
作者
Yanming AN; Zongming FU; Catherine FENSELAU;
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正文语种 eng
中图分类分析化学;一般工业技术;
关键词
proteomics; isoelectrofocusing; nuclear proteins; multi-compartment electrolyzer; MALDI-TOf;

机译：蛋白质组学;等电聚焦;核蛋白;多室电解槽;MALDI-TOf;

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7. Evaluation of Solution Phase Isoelectrofocusing as Part of Proteomics Strategies [O] . Yanming AN, Zongming FU, Catherine FENSELAU 2005

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Evaluation of Solution Phase Isoelectrofocusing as Part of Proteomics Strategies

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