...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of the Mass Spectrometry Society of Japan >Effective Extraction and Analysis for Lysophosphatidic Acids and Their Precursors in Human Plasma Using Electrospray lonization Mass Spectrometry
【24h】

Effective Extraction and Analysis for Lysophosphatidic Acids and Their Precursors in Human Plasma Using Electrospray lonization Mass Spectrometry

机译：电喷雾电离质谱法有效提取和分析人血浆中的溶血磷脂酸及其前体

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

In the current studies, we examined the molecular species of lysophosphatidic acids (lysoPAs) and their precursors in human plasma. We first examined the extraction of phospholipids under highly acidic conditions, which have been reported to allow high recovery of lysoPAs. Capillary liquid chromatography/electrospray ionization mass spectrometry (LC/ESIMS) revealed a variety of phospholipids, including lysophosphatidyl-ethanolamines (lysoPEs), lysoPAs, and lysophosphatidylmethanols (lysoPMEs). However, we found that most of lysoPMEs and part of lysophosphatidylcholines (lysoPCs), lysoPEs, and lysoPAs were artifacts generated by hydrolysis under acidic extraction conditions. In contrast, a solid phase method was effective for the extraction of lysophospholipids, including lysoPAs. Analysis of the solid phase-extracted lipids obtained from plasma by LC/ESIMS (C18 column), followed by neutral loss scanning and product ion scanning, indicated that acyl 16:0, 18 : 0, 18 :1, 18 : 2, 20 : 4, and 22 : 6 lysoPEs are present in human plasma. Furthermore, following incubation of plasma at 37°C, there was an increase in lysoPAs. On the other hand, the levels of some molecular species of lysoPCs and lysoPEs were reduced, indicating that lysoPCs and lysoPEs are precursors of lysoPAs in human plasma.
机译：在当前的研究中，我们检查了人血浆中溶血磷脂酸（lysoPAs）及其前体的分子种类。我们首先检查了在高酸性条件下提取磷脂的方法，据报道该方法可实现lysoPAs的高回收率。毛细管液相色谱/电喷雾电离质谱（LC / ESIMS）显示了各种磷脂，包括溶血磷脂酰乙醇胺（lysoPEs），溶血素PA和溶血磷脂酰乙醇（lysoPME）。但是，我们发现大多数lysoPME和部分溶血磷脂酰胆碱（lysoPC），lysoPE和lysoPA是在酸性萃取条件下水解产生的假象。相反，固相方法对溶血磷脂（包括lysoPAs）的提取是有效的。通过LC / ESIMS（C18色谱柱）分析血浆中提取的固相脂质，然后进行中性损失扫描和产物离子扫描，结果表明酰基16：0、18：0、18：1、18：2、20人血浆中存在4：4和22：6 lysoPE。此外，在37°C下孵育血浆后，lysoPAs有所增加。另一方面，lysoPC和lysoPEs的某些分子种类的水平降低，表明lysoPCs和lysoPEs是人血浆中lysoPAs的前体。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号