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首页> 外文期刊>Journal of the Mass Spectrometry Society of Japan >Optimization of Matrix and Amount of Ammonium Chloride Additive for Effective lonization of Neutral Oligosaccharides as Chloride Ion Adducts in Negative-Mode MALDI-TOF Mass Spectrometry
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Optimization of Matrix and Amount of Ammonium Chloride Additive for Effective lonization of Neutral Oligosaccharides as Chloride Ion Adducts in Negative-Mode MALDI-TOF Mass Spectrometry

机译：负模式MALDI-TOF质谱法中中性寡糖作为氯离子加合物的有效电离的基质和量的氯化铵添加剂的优化

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The combination of harmine and ammonium chloride is a useful matrix in detecting the chloride ion adducts of neutral oligosaccharides in negative-ion MALDI-TOF mass spectra. Sandwich method is the best way to prepare the mixed crystals of matrix, analyte, and additive on the target. Changing the molar ratio of ammonium chloride additive from 0 to five times the amount of harmine matrix, the relative intensity of the chloride ion adducts of the analyte molecules to the deprotonated matrix molecular ion ([Analyte + Cl]~-/ [Matrix — H]~-) increases logarithmically. A ratio of several times the amount of additive to matrix works most effectively in the formation and detection of the chloride ion adducts of the neutral oligosaccharides as opposed to anamount of additive that is on the same order as the analytes.
机译：harmine和氯化铵的组合是检测负离子MALDI-TOF质谱图中中性寡糖的氯离子加合物的有用基质。三明治法是在目标上制备基质，分析物和添加剂的混合晶体的最佳方法。将氯化铵添加剂的摩尔比从0更改为harmine基质的量的5倍，即分析物分子与去质子化基质分子离子的氯离子加合物的相对强度（[Analyte + Cl]〜-/ [Matrix-H ]〜-）对数增加。相对于与分析物相同数量的添加剂数量，添加剂与基质的比例是形成中性寡糖的氯离子加合物的最有效方法。

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