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首页> 外文期刊>Journal of Thrombosis and Thrombolysis >Aldehyde dehydrogenase activity allows reliable EPC enumeration in stored peripheral blood samples
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Aldehyde dehydrogenase activity allows reliable EPC enumeration in stored peripheral blood samples

机译：醛脱氢酶活性可对储存的外周血样品进行可靠的EPC计数

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Background Interest in the biology of endogenous progenitor cells (EPCs) continues to grow as evidence of their role in vascular repair mounts. EPC enumeration requires specialized laboratory techniques and is performed immediately after sample acquisition, limiting the clinical contexts in which EPC enumeration can be performed and the ability to increase sample sizes through multi-center participation. Methods We compared the numbers of EPCs enumerated in samples processed immediately after acquisition (n = 36) with EPCs enumerated in specimens stored for 24 hours or after cryopreservation of mononuclear cells (MNC) using two EPC identification strategies: cell surface marker expression (CD133/CD34) and aldehyde dehydrogenase activity (ALDHbr cells). Results EPCs assessed in fresh samples correlated with EPCs enumerated after whole blood storage (r = 0.699 for CD133+CD34+ cells, r = 0.880 for ALDHbr cells, P < 0.005 and P < 0.0001, respectively) or mononuclear cryopreservation (r = 0.590 for CD133+CD34+ cells, r = 0.894 for ALDHbr cells, P < 0.0001 for each); however, correlation based on assessment of ALDHbr cells was higher (P < 0.0003 for comparison of correlation coefficients). Initial results from a multi-site clinical trial suggest that EPC enumeration after mononuclear cell cryopreservation is feasible. Conclusion EPC analysis based on ALDH activity is reproducible, even after extended whole blood storage or MNC cryopreservation.
机译：背景技术内源性祖细胞（EPC）在生物学中的兴趣不断增长，因为它们在血管修复支架中的作用得到了证明。 EPC枚举需要专门的实验室技术，并且在获取样本后立即进行，这限制了可以进行EPC枚举的临床环境以及通过多中心参与增加样本量的能力。方法我们采用两种EPC识别策略，将采集后立即处理的样本（n = 36）中的EPC数量与保存24小时或冷冻保存单核细胞（MNC）的样品中枚举的EPC数量进行比较：细胞表面标志物表达（CD133 / CD34）和醛脱氢酶活性（ALDH br 细胞）。结果新鲜样品中评估的EPC与全血储存后的EPC相关（CD133 + CD34 + 细胞r = 0.999，ALDH br 0.880 sup>细胞，分别为P <0.005和P <0.0001）或单核冷冻保存（对于CD133 + CD34 + 细胞，r = 0.590，对于ALDH ，r = 0.894 br 单元格，每个P <0.0001）；然而，基于ALDH br 细胞评估的相关性更高（相关系数的比较P <0.0003）。来自多站点临床试验的初步结果表明，单核细胞冷冻保存后进行EPC枚举是可行的。结论即使在延长全血储存或MNC冷冻保存后，基于ALDH活性的EPC分析仍可重现。

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